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《不同麻醉剂对刺参幼参麻醉效果的比较研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中国震亏通提2014,30(2):95—99ChineseAgriculturalScienceBulletin不同麻醉剂对刺参幼参麻醉效果的比较研究何舟,宋坚,常亚青,程龙,宁军号(大连海洋大学/农业部北方海水增养殖重点实验室,辽宁大连116023)摘要:为了找到一种可以准确测量活体刺参体长的方法,研究了不同浓度及不同药浴时间条件下,硫酸镁、丁香酚和乙二醇苯醚等三种麻醉剂对仿刺参(Apostichopusjaponicus)幼参(1.3815~0.2138)g的麻醉效果。试验结果显示:(1)乙二醇苯醚的麻醉效果最差,丁香酚在浓度较
2、小f<0.3mol/L)时有一定的麻醉效果。当浓度过大时,二者都会对刺参造成伤害。硫酸镁在浓度为0.3~0.4mol/L时麻醉效果最好,麻醉时间较快(约30min)且麻醉率和苏醒率均达100%。(2)在0.3~0.4mol/L的硫酸镁溶液中药浴1h左右的刺参,其体长与药浴前的体长变化差异极显著,而药浴l、1.5、2h的刺参个体间体长变化不显著。以上结果表明:0.3~0.4mol/L的硫酸镁溶液对刺参的麻醉效果最好,既不影响刺参的正常生长又可准确测量其自然生长状态长度。通过本研究确定硫酸镁是刺参的最佳麻醉剂,用于刺参体长测量的最佳浓度
3、和麻醉时间分别为0.3~0.4mol/L和1h。关键词:刺参;硫酸镁;丁香酚;乙二醇苯醚中图分类号:$917.4文献标志码:A论文编号:2013—2524AComparativeStudyoftheAnestheticEfectofDiferentAnestheticOUJuvenileSeaCucumber(Apostichopusjaponicus)HeZhou,SongJian,ChangYaqing,ChengLong,NingJunhao(DalianOceanUniversity~KeyLaboratoryofMaric
4、ultureandStockEnhancementinNorthChina’sSea,MinistryofAgriculture,DalianLiaoning116023)Abstract:Thecurrentstudyaimedtofindthebestanestheticforaccuratebodylengthmeasurement.Theanestheticeffectsofmagnesiumsulfate,eugenolandethyleneglycolphenylethercEPH)onjuvenileseacucumb
5、er(Apostiehopusjaponicus)(1.3815_+0.2138)g,(Mean_+SD)weretestedatdifferentconcentrationsandtreattimes.Theresultsshowedthat:f11theanestheticeffectofEPHwasminusandtheeugenolhadamoderateanestheticeffectonlyatlowconcentrationf≤0.3mol/L).Bothreagentscausedsignificantsideeff
6、ectsatincreasedconcentrations.TheanestheticeffectofmagnesiumsulfatewasbestattheconcentrationofO.3-0.4mol/L.Theanesthetictookeffectsabouthalfanhourafter~eatment.Theanestheticandrecoveryratesallreachedto100%.(2)Individualsweretreatedbymagnesiumsulfateseawatersolutionatco
7、ncentrationsof0.3mol/Land0.4mol/Lrespectively.Thebodylengthswereextremelysignificantlylongerthanthosebeforetreatment,andwerenotsignificantlydifferentamong1h,1.5hand2htreatmentsforbothconcentrations.Theseaboveresultsindicatedthatthebestworkconcentrationofmagnesiumsulfat
8、eforA.japonicuswasO.3-0.4mol/L.Thesuggestedconcentrationdidn’taffectthegrowthofseacucumbersandgreatlyimprovedtheaccur
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