P物质对新生大鼠心肌细胞缺氧复氧损伤的影响.pdf

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1、中华麻醉学杂志2014年7月第34卷第7期ChinJAnesthesiol，July2014，Vo1．34，No．7873·重症医学P物质对新生大鼠心肌细胞缺氧复氧损伤的影响赵鑫陈璐郭政【摘要】目的探讨P物质对新生大鼠心肌细胞缺氧复氧损伤的影响。方法新生sD大鼠，1～3日龄，处死后取心室肌组织，分离心肌细胞，接种于6孔培养板中，培养72h。采用随机数字表法，将心肌细胞分为4组(n=3)：对照组(C组)常规培养6h；缺氧复氧组(A／R组)进行缺氧3h，复氧2h；P物质组(sP组)给予终浓度为10一mol／L的P物质孵育lh，行缺氧3h，复氧2h；P物质+D．

2、sP组(sP+D．SP组)给予终浓度为10。mol／L的P物质和终浓度为10mol／L的速激肽1型受体拮抗剂D．SP孵育1h，行缺氧3h，复氧2h。于复氧结束时，测定心肌细胞凋亡率和乳酸脱氢酶(LDH)活性。结果与c组比较，A／R组、sP组和sP+D—sP组细胞凋率和LDH活性升高(P<0．01)；与A／R组比较，sP组和sP+D．sP组细胞凋亡率和LDH活性降低(P<0．01)；与sP组比较，sP+D—SP组细胞凋亡率和LDH活性升高(P<0．01)。结论P物质可减轻新生大鼠心肌细胞缺氧复氧损伤，其机制与激活速激肽1型受体有关。【关键词】P物质；肌细胞，

3、心脏；细胞低氧；氧；婴儿，新生EfectsofsubstancePonanoxia／reoxygenationinjurytocardiomyocytesofneonatalratsZhaoXin，ChenLu，GuoZheng．DepartmentofAnesthesiology，ShanxiMedicalUniversity，Taiyuan030001，ChinaCorrespondin~author：GuoZheng，DepartmentofAnesthesiology，ShanxiMedicalUniversitySecondHosptialofS

4、hanxiMedicalUniversity，Taiyuan030001，China，Email：guozheng713@163．corn【Abstract】ObjectiveToinvestigatetheeffectsofsubstancePonan0xia／re0xygenati0n(A／R)injurytocardiomyocytesofneonatalrats．MethodsCardiomyocytesofneonatalratswereisolatedfromSprague—Dawleyrats，aged1-3days，andwerecultu

5、redin6-wellplatesfor72h．Thecardiomyocyteswerethenassignedinto4groups(n=3each)usingarandomnumbertable：controlgroup(groupC)，A／Rgroup，substancePgroup(groupSP)andsubstanceP+D—SPgroup(groupSP+D—SP)．Thecellswereculturedroutinelyfor6hingroupCandthecellswereexposedto99．9％N2inanincubatorat

6、37ccfor3hfollowedbyreoxygenationfor2hintheothergroups．ThecellswereincubatedwithsubstancePwiththefinalconcentrationof10～mol／Lfor1hbeforean0xiaingroupSP．Thecellswereincubatedfor1hwithsubstancePwiththefinalconcentrationof10一mol／LandD。SP(aspecificantagonistofneurokinin．1receptor)witht

7、hefinalconcentrationof10“mol／LbeforeanoxiaingroupSP+D—SP．Theapoptosisrateandlactatedehydrogenase(LDH)activityweredetectedattheendofreoxygenationusingTUNELassayandLDHassaykit，respectively．ResultsComparedwithCgroup，theapoptosisrateandLDHactivityweresignificantlyincreasedinA／R．SPandS

8、P+D—SPgroups．ComparedwithA／Rgroup