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1、江西医学检验2007年6月第25卷第3期·201··论著·新型化学氧化法测定胆红素试剂的研制袁水斌1徐光明1邓荣春2陈江平1文玲梅3中图分类号R446.11+2,Q592.1文献标识码A文章编号1008-0023(2007)03-0201-06[摘要]目的探讨建立一种新型化学氧化法测定血清总胆红素和直接胆红素的新方法。方法采用化学氧化法测定血清胆红素反应前后吸光度的变化,与标准品相比较,求得标本中总胆红素和直接胆红素的浓度。结果TBIL精密度试验批内高低浓度CV值为0.65%～1.56%;DBIL为0.82%～1.93%。批间TBIL为3.5%、1.71%及1.24%;DBIL
2、批间为4.38%、2.77%及1.60%。线性范围:TBIL可达686μmol/L;DBIL可达400μmol/L;总胆回收率101.8%～107.6%,平均104.5%.DBIL回收率为99.1%～100.5%,平均为100.2%。与其他相关试剂比较TBILγ=0.9973～0.9992;DBILγ=0.9962～0.9994。溶血、脂浊和维生素C干扰试验表明:标本中Hb小于2.7g/L,TG小于5.94mmol/L。VitC小于0.1g/L,对TBIL和DBIL的测定基本无干扰。结论本试剂方法简便,结果准确、线性范围宽,试剂稳定,价格低廉。完全可用于检测临床标本。[关键词]
3、化学氧化法;总胆红素;直接胆红素;试剂研制DevelopmentofthereagentswithnewchemicaloxidationmethodformeasuringserumbilirubinYUANShuibin,XUGuangming,DENGRongchun,etal.JiangxiProvin-cialTumorHospital,Nanchang330029,China[Abstract]ObjectiveToestablishanewchemicaloxidationmethodformeasuringserumbilirubin.MethodsChemic
4、aloxidationmethodwasappliedfordetectingserumtotalbilirubin(TBIL)andcholebilirubin(DBIL)concentrationsaccordingtotheabsorbancechanges,andtheresultswereanalysedcomparingwiththestandardcontrols.ResultsTheintra-assayCVsofTBILwere0.65%and1.56%(n=15),withthemeanvaluesof29.35and221.7μmol/Lrespectiv
5、ely,whilethatofDBILwere0.82%and1.93%(n=15),withthemeanvaluesof9.70and119.76μmol/Lrespectively.Theinter-assayCVsofTBILwere3.50%,1.71%and1.24%(n=20),withthemeanvaluesof15.67,214.47and564.98μmol/Lrespectively,whilethatofDBILwere4.38%,2.77%and1.60%(n=20),withthemeanvaluesof5.18,76.12and251.83μmo
6、l/Lrespectively.Thelinearrangeswereupto686μmol/LforTBILand400μmol/LforDBILrespestively.Theaveragerecoverieswere104.5%forTBILand100.2%forDBIL.ThenitritemethodwaswellcorrelatedwithVanadatemethodandDiazomethod.Whenhemoglobinlevelinserumwasunder2.7g/L,Triglyceridesunder5.94mmol/L,orVitaminCunder
7、0.1g/L,theresultsshowednosignificantinterferenceformeasuringbilirubin.Con-clusionsThisnewchemicaloxidizationmethodissimpleandstable,anditcanbeusedinroutinedetectionsofserumtotalTBILandDBIL.[Keywords]Chemicaloxidationmethod;Totalbilirubin;Cholebilir
