鼠尾悬挂试验在大鼠急性坏死性胰腺炎中的实验意义.doc

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1、鼠尾悬挂试验在大鼠急性坏死性胰腺炎中的实验意义【摘要】目的:探讨鼠尾悬挂试验(TST)在大鼠急性坏死性胰腺炎中的实验意义。方法:SD大鼠被分为三组：急性坏死性胰腺炎组(A组，n二8)、急性坏死性胰腺炎鱼油干预组(F组，n=8)和对照组(C组，1尸8)。观察各组大鼠胰腺组织病理变化及TST静止时间，同时测定各组5d后血清SOD、GSH-Px活力和淀粉酶浓度。结果M组、F组TST静止时间各时间段均显著高于C组(P<0.01)；自第2天起F组的数值均小于A组，其中第4、第5天的数值差异有显著性(

2、分别为P<0・05、P<0.01)o病理学检查提示F组的炎性细胞浸润显著低于A组(P<0.01)oA组SOD、GSH-Px显著低于C组(P<0.01),而F组SOD、GSH-Px显著高于A组(P01)。TST静止时间和病理学改变、SOD、GSH-Px活力具有相关性(P<0.01)o结论:TST静止时间在一定程度上反映了急性坏死性胰腺炎的严重程度；鱼油在改善急性坏死性胰腺炎大鼠的氧化应激指标的同时，对大鼠的TST静止时间产生影响。【关键词】胰腺炎急性坏死性鼠尾悬挂实验

3、超氧化物歧化谷胱甘肽过氧化酶Abstract:Objective:Toinvestigatethesignificanceofthetai1suspensiontestinratswithacutenecrotizingpancreatitis(ANP).Methods:ThemaleSprague-Dawley(SD)ratsweredividedintothreegroups:ANPgroup(groupA,n=8),ANPwithfishoi1treatmentgroup(groupF,n

4、二8)andcontrolgroup(groupC,n=8).Theimmobilitytimeinthetailsuspensiontestandthegradeoftheseverityofpancreatitiswereobserved・Afterexperimentstartedforfivedays,theactivityofserumantioxidantenzymes(SOD,GSH-Px)andtheconcentrationofserumamylaseinratsofeachg

5、roupweremeasuredrespectively.Results:TheimmobilitytimeinthetailsuspensiontestofgroupAandFweresignificantlylongerthanthatofgroupC(P&It;0・01).Sincetheseconddayaftertheexperimenthasstarted,theimmobilitytimeinthetailsuspensiontestofgroupFwasshorterthanth

6、atofgroupA.However,onlyonthe4thandSthday,thisdifferenceshowedstatisticsignificance(P&It;0.05andP<0・01respectively)・HistopathologicalanalysissuggestedtheinfiltrationofthatinflammatorycellsingroupFwassignifleant1yweakerthanthatofgroupA(P&It;0・01)・Se

7、rumglutathioneperoxidase(GSH-Px)andsuperoxidedismutase(SOD)levelingroupAweresignificantlylowerthanthatingroupC(P&It;0.01),andtheseparametersofgroupFweresignificantlyhigherthanthoseofgroupA(P&It;0.01).Thedurationofimmobilityinthetai1suspensiontestwasr

8、elatedtothehistopathologicalchangesandtheactivityofserumSODandGSH-Px.Conclusion:ThedurationofimmobilityinthetailsuspensiontestiscorrelatedwiththeseverityofANP,andfishoilcanaffecttheANPrats?behaviour,andimprovestheseverityofacutenecrotizingpancreatiti