制备肿瘤血管生成双靶点RGD10-NGR9-超顺磁性纳米氧化铁-论文.pdf

《制备肿瘤血管生成双靶点RGD10-NGR9-超顺磁性纳米氧化铁-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。

1、中国医学影像技术2013年第29卷第9期ChinJMedImagingTechnol，2013，Vol29，No9·：·实验研究PreparationofI】【P3integrinandaminopeptidaseNdual—targetingRGD10_-NGR9-。superparamagneticironoxideWUQiong—ya，SHIJing—run，ZHANGJie。，ZHANGLin～qian，ZHAOYin—min。，TANGLiang。，CHENYun，HEXiao—don

2、g，LIUHui，SUBo。(1．DerpartmentofRadiationOncology，2．DepartmentofRadiology，3．CentralLaboratory，ShanghaiPulmonaryHospital，TongJiUniversity，Shanghai200433，China；4．SchoolofMaterialsScienceandEngineering，EastChinaUniversityofScienceandTechnology，Shanghai200

3、237，China)[Abstract]ObjectiveToconstructangiogenesis—。specificRGD10—。NGR9dual’。targetingsuperparamagneticironoxidenan—-oparticies，andtoobservethephysicalproperties．MethodsDual—targetingpeptidesRGD10一NGR9weredesignedandsyn—thesized．Fluorescencelabeled

4、RGD10NGR9peptidesweresynthesizedbyincorporatingflouresceiniso—thiocyanate(FITC)intopeptides．Humanumbilica1veinendotheliacells(HUVEC)wereincubatedwithFITClabeledpeptidesandsubse—quentlytestedbyinvertedmicroscope．Ultrasmallsuperparamagneticironoxide(US

5、PIO)nanoparticleswithnarrowsizedistributionhavingaveragediametersof8—12nmweresynthesizedbyco—precipitationchemicalmethod．andthesurfacewasmodifiedtobehydrophilicbycoatingwithdextran．Then，dextranwasoxidizedandcrosslinkedtoUSPI()bypropyl—eneoxide，andthe

6、hybridpeptidesRGD10一NGR9werefinallyconjugatedtothesurfaceofUSPIOinSchiffbaseswithdex—tran．Themorphologyofpreparednanoparticleswasobservedunderelectronmicroscope．Themeanparticlesizeandsizedistributionwereobservedbydynamiclightscattering．Thesurfacecoat

7、ingmaterialsweredeterminedbyinfraredspec—trometer．Zetapotentialwastestedandmagneticintensitywasexaminedbyvibratingsamplemagnetometer．ResultsInvitrostudiesdemonstratedabetterbindingaffinityofFITC-RGD10一NGR9toHUVEC．RGD10一NGR9targetedUSPIOnanoparticlesw

8、eresuccessfullyconstructed．Thetransmissionelectronmicroscopyofthenanoparticleswashomogeneousinsizeandroundinshape，withaveragediametersof30nm．ThestablepropertiesweretestedwithZetapotentialof一60．9mVandmagneticintensityof53．54emu／gunder70~C．ConclusionRG