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时间:2020-04-25
《制备肿瘤血管生成双靶点RGD10-NGR9-超顺磁性纳米氧化铁-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、中国医学影像技术2013年第29卷第9期ChinJMedImagingTechnol,2013,Vol29,No9·:·实验研究PreparationofI】【P3integrinandaminopeptidaseNdual—targetingRGD10_-NGR9-。superparamagneticironoxideWUQiong—ya,SHIJing—run,ZHANGJie。,ZHANGLin~qian,ZHAOYin—min。,TANGLiang。,CHENYun,HEXiao—don
2、g,LIUHui,SUBo。(1.DerpartmentofRadiationOncology,2.DepartmentofRadiology,3.CentralLaboratory,ShanghaiPulmonaryHospital,TongJiUniversity,Shanghai200433,China;4.SchoolofMaterialsScienceandEngineering,EastChinaUniversityofScienceandTechnology,Shanghai200
3、237,China)[Abstract]ObjectiveToconstructangiogenesis—。specificRGD10—。NGR9dual’。targetingsuperparamagneticironoxidenan—-oparticies,andtoobservethephysicalproperties.MethodsDual—targetingpeptidesRGD10一NGR9weredesignedandsyn—thesized.Fluorescencelabeled
4、RGD10NGR9peptidesweresynthesizedbyincorporatingflouresceiniso—thiocyanate(FITC)intopeptides.Humanumbilica1veinendotheliacells(HUVEC)wereincubatedwithFITClabeledpeptidesandsubse—quentlytestedbyinvertedmicroscope.Ultrasmallsuperparamagneticironoxide(US
5、PIO)nanoparticleswithnarrowsizedistributionhavingaveragediametersof8—12nmweresynthesizedbyco—precipitationchemicalmethod.andthesurfacewasmodifiedtobehydrophilicbycoatingwithdextran.Then,dextranwasoxidizedandcrosslinkedtoUSPI()bypropyl—eneoxide,andthe
6、hybridpeptidesRGD10一NGR9werefinallyconjugatedtothesurfaceofUSPIOinSchiffbaseswithdex—tran.Themorphologyofpreparednanoparticleswasobservedunderelectronmicroscope.Themeanparticlesizeandsizedistributionwereobservedbydynamiclightscattering.Thesurfacecoat
7、ingmaterialsweredeterminedbyinfraredspec—trometer.Zetapotentialwastestedandmagneticintensitywasexaminedbyvibratingsamplemagnetometer.ResultsInvitrostudiesdemonstratedabetterbindingaffinityofFITC-RGD10一NGR9toHUVEC.RGD10一NGR9targetedUSPIOnanoparticlesw
8、eresuccessfullyconstructed.Thetransmissionelectronmicroscopyofthenanoparticleswashomogeneousinsizeandroundinshape,withaveragediametersof30nm.ThestablepropertiesweretestedwithZetapotentialof一60.9mVandmagneticintensityof53.54emu/gunder70~C.ConclusionRG
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