不同诱导子对黄芪悬浮培养细胞中黄芪多糖积累的影响-论文.pdf

《不同诱导子对黄芪悬浮培养细胞中黄芪多糖积累的影响-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、安徽农业科学．JournalofAnhuiAgIi．Sci．2014。42(26)：8954—8956，8959责任编辑黄小燕责任校对况玲玲不同诱导子对黄芪悬浮培养细胞中黄芪多糖积累的影响杨静，剥、皓(天津渤海职业技术学院，天津300402)摘要[目的]研究水杨酸、茉莉酸甲酯、硝酸银、高糖、高盐、超声等诱导子对黄芪细胞生长和黄芪多糖合成的影响。[方法]向细胞悬浮液中加入各种诱导子或对细胞悬浮液进行超声处理，测定细胞干重增殖倍数和黄芪多糖的合成量。[结果]加入10．0ms／L水杨酸可使黄芪多糖的含量为对照组的2．o

2、4倍；添加较低浓度的茉莉酸甲酯浓度(1．0ms／L)使黄芪多糖的含量达48．974ms／g，比对照提高了2．50倍；添加4mg／L浓度的硝酸银能使黄芪多糖含量提高1．6o倍；高糖和高盐胁迫处理均能诱导黄芪多糖的合成。高糖处理能使黄芪多糖的产量比对照提高了1．92倍。低剂量的超声刺激对黄芪细胞生物量的积累有显著的刺激作用。[结论]诱导子的添加可有效促进黄芪悬浮培养细胞中黄芪多糖的积累。关键词诱导子；黄芪多糖；积累；细胞悬浮培养；黄芪中图分类号S567文献标识码A文章编号05l7—6611(2014)26—08954

3、—03EfectofDifferentElicitorsonAccumulationofAstragalusPolysaccharidesintheSuspensionCultureCellofAsWagalusmembrana-CelYANGJjngetaI(TianjinBohaiVocationalandTechnicalCollege，Tianjin300402)Abstract[Objective]Toinvestigatetheefectsofdiferentelicitors(salicylicac

4、id，methyljasmonate，silvernitrate，highsucrose，hi【ghsalt，ultrasonicstimulation)onthecellgrowthandinducibleformationofastragaluspolysaceharidesinAstragalusmembranac~ll$cens．[Meth一0d]Thesuspensionculturecellsweretreatedwiththeelicitorsorultrasonicstimulation．Then

5、thecellgrowthrateandtheastragaluspolysac．ehafidescontentinAstragalusmembranaceucelsweredetermined．[Result]10．0mg／Lsalicylicacidresultedinanincreaseofastragaluspolysaccharidescontentbyabout2．04times．Withtheadditionofmethyljasmonate，thehighestcontentofastragalu

6、spolysacchafidesreached48．974mg／g．anditwas2．50timeshigherthantheeonto1．eadditionof4mg／Lsilvernitratepromoteastragaluspolysaccharidescontentby1．60times．Boththehighsucroseandhighsalttreatmentcouldinducethesynthesisofastragaluspolysaceharides．Withthehighsucroset

7、reatment。theastragatuspolysaeehafidescontentwasincreasedby1．92times．Lowdoseofultrasonicstimulationcansignificantlystimulatethecellgrowth．[Conclusion]eadditionoftheelicitorswasefectivetoenhancetheformationofastragaluspolysaccharidesinAstragalusmembranaceuscels

8、．KeywordsElicitors；Astragaluspolysaeehafides；Accumulation；Suspensionculturecell；Astragalusmembranaceus黄芪(Astragalusmembranaceus(Fisch．)Bunge．)是豆科多芪细胞大规模培养奠定基础。年生草本植物，根是中药的中药材，主产于黑龙江、内蒙和山1