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《不同浓度艾烟干预12周对大鼠肺与骨骼肌的影响-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、环球中医药2014年9月第7卷第9期GlobalTraditionalChineseMedicine,September2014,Vo1.7,No.9661·论著·不同浓度艾烟干预12周对大鼠肺与骨骼肌的影响黄剑韩丽林岷瑜白桦杨佳王磊崔莹雪刘钧天黄畅赵百孝【摘要】目的观察亚急性毒理实验中不同浓度艾烟对大鼠血清瘦素、肺、膈肌和趾长伸肌腱的影响,为艾烟的安全性评价提供参考。方法将40只雌雄Wistar大鼠随机分为四组,每组10只,雌雄各半,分别暴露于0mg/m、2.5~3.5mg/m、8.0—10.0mg/m、27.0—2
2、9.0mg/m的艾烟浓度,200min/day,5次/周,12周后取材,采用酶联免疫吸附双抗体夹心法(enzymelinkedimmunosor-bentassays,ELISA)检测不同浓度组大鼠血清瘦素、膈肌、趾长伸肌腱肿瘤坏死因子·(tumornecrosisfactor,TNF.n)、白介素一8(interleukin.8,IL.8)的含量,并进行肺和骨骼肌的组织病理学检查。结果低、中、高浓度组血清瘦素、膈肌和趾长伸肌腱TNF—n、IL一8含量与空白对照组无显著差异(P>0.05),各组肺、膈肌和趾长伸肌腱病理
3、未见显著改变。结论亚急性毒理实验低、中、高浓度艾烟对大鼠肺、骨骼肌未见明显损伤。【关键词】艾烟;毒理;慢性阻塞性肺病;骨骼肌;瘦素【中图分类号】R245.81【文献标识码】Adoi:10.3969/j.issn.1674—1749.2014.09.00112weeksofmoxasmokeinterventiononrats’lungandskeletalmuscles:anexperimentalstudyHUANGJian,HANLi,LINMin—Yu,etaLSchoolofAcupuncture—Moxibu
4、stionandTui—Na,BeijingUniver—sityofChineseMedicine,Beijing100029,ChinaCorrespondingauthor:ZHAOBai—xiao,E—mail:baixiaolO0@岫sina.com【Abstract】ObjectiveToinvestigatethesubacutetoxicitiesofmoxasmokeonthesernmleptin,lung,diaphragmandextensordigitorumlongustendoninrat
5、s,SOastoprovidereferenceforthesafetyeval—uationofmoxasmoke.Methods20maleand20fe:maleWistarratswererandomlydividedintofourgroups(n=10).Theexperimentalgroupswererespectivelyexposedto0mg/m,2.5—3.5mg/m,8.0—10.0mg/mand27:0—29.0mg/mconcentrationsofmoxasmoke,200min/day
6、,5times/week.Theratsweresacrificedafter12weeks.ELISAwasusedtodetectthelevelsofserumleptin,andTNF一andIL-8ofthediaphragmandextensordigitorumlongustendon.Histopathologicalexaminationwasalsoperformedonthelung,diaphragmandextensordigitorumlongustendon.ResultsNosignif
7、icantdifferencewasobservedinthelevelsofserumleptin.andTNF一andIL-8ofthediaphragmandextensordigitorumlongustendonbetweenthecontrolgroupandexperimentalgroups(P>0.05).Histopath0localexamina—tionofthelung,diaphragmandextensordigitorumlongustendondidnotpresentsignific
8、antchangeseither.ConclusionThesubacutetoxicitystudyshowsthatlow,mediumandhighconcentrationsofmoxasmokedoesnotcauseobviousdamagetorats’lungandskeletalmuscles.【Keywords
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