Folin-Ciocalteu法测定山茱萸果核水溶性提取物多酚含量的研究-论文.pdf

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1、·84-JHenanUnivSciTech(MedSci)June2014Vo1．32No．2Folin·Ciocalteu法测定山茱萸果核水溶性提取物多酚含量的研究杨晖，和素娜’，杜景霞，李艳，路西明，王建刚，吕茂平摘要：目的以没食子酸为标准物质，研究Folin—Ciocalteu法测定山茱萸果核水溶性提取物多酚含量的优化条件。方法以吸光度为评价指标，分别研究了检测波长、Folin—Ciocaheu试剂用量、NaCO用量、反应温度及反应时间对测定结果的影响。结果优化测定条件：检测波长760nm，0．4mL样品加入Folin—Ciocaheu试剂1．0mL、

2、10％Na2c0，溶液3．2mL，反应温度35℃，显色反应时间1．0h，吸光度与多酚含量在0—250~g／mL范围内呈良好的线性关系(Y=0．0053x+0．0389，R=0．9993)。方法的加标回收率为98．5％一103．0％，相对标准偏差为1．9％～2．3％(vt=6)。三批山茱萸果核水溶性提取物多酚含量平均值为39．24％。结论Folin．Ciocaheu法在本研究建立的优化测定条件下显色灵敏、准确可靠、稳定性高、简便易行，适用于山茱萸果核水溶性提取物多酚的含量测定。关键词：Folin—Ciocaheu法；山茱萸果核；水溶性提取物；多酚中图分类号：R

3、284．1文献标志码：A文章编号：1672—688X(2014)02—0084—03StudyonDeterminationofPolyphenolsinWater-solubleExtractofCornusOfficinalisKernelsbyFolin-CiocalteuMethodYANGHui，HESu—Ha，DUJing—xia，etal(MedicalCollege，HenanUniversityofScienceandTechnology，Luoyang471003，China)Abstract：ObjectiveTheoptimumcon

4、ditionsofFolin—Ciocalteumethodforthedeterminationofpolyphenolscontentinthewater—solubleextractofcomusofficinaliskernelswasstudiedbyusinggallicacidasthestandardsubstance．MethodsAbsorbencywasadoptedasevaluationindex，factorsthatinfluencedthedetectionperformance，suchasthedetectionwavel

5、ength，theamountofFolin—Ciocalteureagent，theamountofsodiumcarbonate，reactiontimeandreactiontemperaturewereinvestigated．ResultsTheoptimumconditionsforpolyphenolsdeterminationbyFolin—Ciocalteumethodwereasfollows：1．0mLofFolin—Ciocalteureagentwasaddedin1．0mLofsample，andthen3．2mLof10％sod

6、iumcarbonatesolutionwasadded．Afterthecolorationreactionwasfor1．0hat35℃．theabsorbancewasmeasuredat760nm．Undertheoptimumconditions，theabsorbanceincreasedlinearlywiththeconcentrationofgallicacidintherangeof0to250Ixg／mL(Y=0．0053x+0．0389，R=0．9993)．Theaveragespikedrecoverieswere98．5％～103

7、．0％withtherelativestandarddeviationsof1．9％～2．3％(n=6)．Theaveragevalueofpolypherlolscontentinthewater—solubleextractofcornusofieinaliskernelsfromthreebatcheswas39．24％．ConclusionFolin—Ciocalteumethodestablishedinthisstudyissimple，fast，highlysensitiveandverystable．Itissuitabletobeappli

8、edtodeterminepolyphenolsco