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时间:2020-04-20
《顺铂对结直肠癌细胞增殖及侵袭能力的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、638TianjinMedJ,July2014,Vo1.42No.7顺铂对结直肠癌细胞增殖及侵袭能力的影响徐阳【摘要】目的探讨顺铂对人结直肠癌细胞SW480增殖及侵袭力的影响。方法以SW480细胞为研究对象,设定未经处理的SW480细胞为对照组,给予不同浓度顺铂进行不同时间干预,应用MTT法、Transwell侵袭小室、定磷法检测SW480细胞的增殖、侵袭力及Na一K一ATP酶活性。结果生理浓度顺铂(70txmol/L)在48h即可抑制SW480细胞的增殖,与72和96h相比抑制率差异无统计学意义;70mol/L』顷铂处理4
2、8h时,即可降低穿越Matrigel膜基质的细胞数;分别采用17.5、35、70和140gmol/L4个梯度浓度的Nef作用于SW480细胞48h后,35、70和140gmol/L组细胞中Na一K一ATP酶活性均显著升高,且顺铂浓度达70gmol/L时Na一K一ATP酶即可达到较高活性。结论Na一K一ATP酶活性的降低可能导致对结直肠癌细胞增殖和侵袭能力调节作用减弱,可能与结直肠癌细胞SW480耐顺铂有关。【关键词】结直肠肿瘤;顺铂;细胞增殖;肿瘤侵润;抗药性,肿瘤;SW480;Na一K一ATP酶【中图分类号】R349.5,
3、R735.34【文献标志码】A【DOI】10.3969/j.issn.0253—9896.2014.07.004EfectsofCisplatinonProliferationandInvasionofColorectaICancerCellsXUYangDepartmentofColorectalSurgery,TheSecondHospitalofTianjinMedicalUniversity,Tianjin300211,China【Abstract】ObjectiveToinvestigatetheeffectofc
4、isplatinonproliferationandinvasionofcolorectalcancercelllineSW480.MethodsSW480cellswereusedastheresearchobject,anduntreatedSW480cellswereusedasthecontrolgroup.Differentconcentrationsofcisplatinwasgivenatdifferenttimestointervene.TheMTY,Transwellchamberandphos-phate
5、determinationmethodswereusedtodetectproliferation.invasionandNa一K一ATPaseactivityexpressionlevelinSW480cells.ResultsThephysiologicalconcentrationofcisplatin(70~tmol/L)inhibitedtheproliferationofSW480cellsat48h.Therewasnosignificantdifferenceintheinhibitionrateat48hc
6、omparedwith72hand96h.Treatmentwith70gmol/Lcisplatinfor48hreducedthenumberofcellsthroughMatrigelmembranematrix.TheNa一K一ATPaseactivitywassignificantlyincreasedin35,70and140gmol/Lcellsaftertreatmentwith17.5,35,70and140p.mol/LofcisplatininSW480cellsfor48h.andNa一KATPase
7、reachedthehighestlevelat70gmol/Lofcisplatin.ConclusionThedecreasedactivityofNa一K一ATPasemayleadtotheattenuationinproliferationandinvasionofcolorectalcancercells.whichmaybeassociatedwithcisplatinresistanceincolorectalcancercellSW480.【Keywords】colorectalneoplasms;cisp
8、latin;cellproliferation;neoplasminvasiveness;drugresistance,neoplasm;SW480:Na一K一ATPase结直肠癌是目前最常见的恶性肿瘤之一,且发用的影响及Na一K一ATP酶、活.1生和表达的差异。病率有逐年上升趋势。肿瘤的
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