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时间:2020-04-20
《薯莨色素的提取工艺及基本成分和抗菌活性研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、1O6食品与药品FoodandDrug2014年第16卷第2期薯莨色素的提取工艺及基本成分和抗菌活性研究彭建飞,范润珍,李晓菲(1.深圳市计量质量检测研究院,广东深圳518109;2.广东海洋大学理学院,广东湛江524025;3.湛江市农业技术推广中心,广东湛江524038)摘要:目的探讨薯莨色素的提取工艺,研究其基本成分和抗菌活性。方法以60%丙酮为提取剂,料液比(w/v)1:1o,常温浸渍5h,60℃水浴回流3h,提取薯莨色素;测定其水分、灰分、粗蛋白、粗脂肪及粗纤维等含量;并以大肠杆菌、桉树青菇菌、巴氏杆菌、蜡状芽孢杆菌、芽孢杆菌、沙门菌为供试菌,检验提取薯莨色素的抗菌活性。结
2、果薯莨色素提取率达28.6%;薯莨色素基本成分为:水分22.61%,灰分1.61%,粗脂肪2.89%,粗纤维2.80%,粗蛋白3.80%;薯莨色素浓度在0.1%~0.50%范围内对6种供试菌种皆显示了不同程度的抑制效果。结论薯莨色素是具有抗菌活性的天然色素,具有一定的开发价值。关键词:薯莨;色素;提取工艺;抑菌活性中图分类号:11284.1文献标识码:A文章编号:1672.979X(2014)02.0106.04ResearchonExtractionTechnologyofDioscoreaCirrhosaPigmentandItsElementaryComponentsandAn
3、tibacterialandAntifungalActivityPENGJian.fei,FANRun.zhen,LIXiaofei(1.ShenzhenAcademyofMetrology&QualityInspection,Shenzhen518109,China;2.CollegeofScienceofGuangdongOceanUnivercity,Zhanjiang524025,China;3.AgricultureTechnologyPopularizingCenterinZhanj’iangZhanjiang524038,China)Abstract:Objective
4、ToexploretheextractiontechnologyofDioscoreacirrhosapigmentandtoresearchtheelementarycomponentsandantibacterialactivityoftheextractedpigment.MethodsTheDiosoreacirrhosapigmentwasextractedunderthecondition:60%acetonewasusedasextractantwiththesolid.1iquidratio1:10.thesamplewassoakedfor5hatnormaltem
5、perature.thenwasrefluxedfor3hinawaterbathat60℃.TheantibacterialactivityoftheDioscoreacirrhosapigmentonEscherichiacoli,Eucalyptusgreenmushroomfungus,Pasteurellamultocide,Bacilluscereus,GemmabacillusandSalmonellawastested.Thecontentsofelementarycomponentsofthepigmentincludingmoisture,ash,crudefat
6、,crudefiberandcrudeproteinweredetermined.ResultsTheextractionrateofDiosoreacirrhosapigmentreached28.6%.Thecontentsofelementarycomponentswereasfollows:moisture22.61%.ash1.61%,crudefat2.89%,crudefiber2.80%andcrudeprotein3.80%.Intheconcentrationrangeof0.1%-0.50%.thepigmentshoweddiferentdegreeofinh
7、ibitoryefectsonthegrowthoftheabovebacteriaandfungus.ConclusionTheDiosoreacirrhosapigmentwasakindofnaturalpigmentwithantibacterialandantifungalactivityandhasdevelopmentalvalueasnaturaldye.KeyWords:Dioscoreacirrhosa;pigment;extracti
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