资源描述：
《培养局的纯培养（Pure culture of culture board）.doc》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、培养局的纯培养(Purecultureofcultureboard)ExperimentsixpurecultureofmicroorganismsFirst,thepurposeandrequirements1.learnandmastervariousasepticmanipulationtechniques,andusethistechniqueformicrobialdilutionseparation,streakseparationandinoculation.2.microorganismswereseparatedbyplatemar
2、kinganddilution.3.tounderstandthecultureconditionsandculturetimeoffourkindsofmicroorganisms・3.understandtheuniversalityofmicroorganismsandunderstandtheimportanceofasepticmanipulation.Two、experimentalprinciplePureculturetechnologyisthemostbasicoperationtechniqueinthelaboratory,t
3、heexperimentpurpose,mediumtypesanddifferentcontainers,differentinoculationmethodsused,aregoodformicroorganism,therefore,usuallyshouldbecarriedoutafterinoculationdisinfectionasepsisroomorvaccinationboxintheair,atthesametimeduetodifferentmethodsofinoculationusingdifferenttools,of
4、tenwith.Isolationandpurificationofmicroorganisms:isolationofmicroorganismswithspecialfunctions,finestrainsandpurificationofcontaminatedbacteria・Purebreedisolationisoneofthemostimportantbasictechniquesinmicrobiology.Isolation:themethodofpurecultureofasinglestrainfromapromiscuous
5、microbialpopulation・卩unified(pureculture):acellorsporeofabacteriumoracultureisproducedbythedivisionandpropagationofasinglecel1.Themethodsofisolationandpurificationofmicroorganismsarecommon:1.simplesinglecellpickingmethodSinglecellpickingmethodcandirectlygetthepureculture.Itrequ
6、iresspecialmicromanipu1atortoseparatesinglecells,ortodirectlyseparatesporesunderordinarymicroscopyatlowmagnification.2.plateseparationmethodTherearetwokindsofdilutionmethod:pouringmethodandcoatingmethod・Thebacteriaareseparatedbyothermiscellaneousbacteriaormixedbacteriasuspensio
7、n.Themethodiseasytooperateandwidelyusedintheseparationandpurificationofmicroorganisms・Theprincipleincludestwoaspects:(1)selectasuitabletargetformicrobialgrowthconditions,suchasnutrients,pH,temperatureandoxygenrequirementsoraddsomeinhibitorscausedbyonlyconducivetothegrowthofmicr
8、oorganisms,andinhibitionofothermicrobialgrowthenvironm
