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1、学习植物组织培养技术（Learnplanttissueculturetechniques）I.teachingaims1.understandthebasicprinciples,objectives,requirementsandmethodsofplanttissueculturetechniques.2.mastertheoperationskillsofplanttissueculture.Two,referencematerialsMediumpreparation,acommonmediuminpl
2、anttissueculture,istheMSmedium.ThepreparationoftheMSmediumcomprisesthefollowingsteps.PreparationandpreservationofMSculturemediumcontainingnearly30kindsofnutritionalcomponentsofbaseliquor,inordertoavoideverymediumpreparationshouldbecarriedoutontheweighingdoze
3、nsofingredients,canbeculturedinthemediumofvariouscomponents,accordingtotheoriginalamountof20timesor200timesrespectivelyweighing,withconcentratedliquid,thisconcentratedsolutioniscalledculturebaseliquor.Inthisway,eachuse,takethetotalamountof1/20(50mL)or1/200(5
4、mL),dilutewithwater,intotheculturemedium.Thequantitiesofvarioussubstancesrequiredforthepreparationofthemotherliquidoftheculturemediumarelistedforuseinpreparation.Alargenumberofelements(motherliquorI)mg/LNH4NO333000KNO338000CaCl22H2O8800MgSO47H2O7400KH2PO4340
5、0Traceelements(MotherLiquorII)KI166H3BO31240MnSO44H2O4460ZnSO47H2O1720Na2MoO42H2O50CuSO45H2O5CoCl26H2O5Ironsalt(motherliquorIII)FeSO47H2O5560Na2-EDTA2H2O7460Organicingredients(motherliquorIV)IVAInositol20000IVBNiacin100Pyridoxinehydrochloride(vitaminB6)100Th
6、iaminehydrochloride(vitaminB1)100Glycine400Theamountofallthenutrientsmentionedabovewas200timesofconcentratedliquidexceptformotherliquor,whichwas20timesconcentratedliquid.Eachofthesemotherliquidsshallbeindividuallyseparatedinto1Lstocksolution.Thepreparationme
7、thodofmotherliquor,liquorandliquorare:IIIVweighingseveralcomponentseachinthemotherliquorafterrespectivelywithdistilledwatertodissolveasmallamount,andthentheyareimmiscible,thefinalvolumeto1L.Thepreparationmethodis:LiquorIIIwillbecalledFeSO47H2OandNa2-EDTA2H2O
8、respectivelyin450mLdistilledwater,heatingandstirringtodissolvethem,thenthetwosolutionsaremixed,andwillbeadjustedtopH5.5,thefinalvolumeto1L,storedinbrownglassbottle.Afterallthemotherliquorhasbeen
