植物组织培养中灭菌和无菌操作的几个问题（several problems of sterilization and aseptic operation in plant tissue culture）

《植物组织培养中灭菌和无菌操作的几个问题（several problems of sterilization and aseptic operation in plant tissue culture）》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、植物组织培养中灭菌和无菌操作的几个问题（Severalproblemsofsterilizationandasepticoperationinplanttissueculture）SeveralproblemsofsterilizationandasepticoperationinplanttissuecultureZhuguanglianBeijinguniversitylifesciencescollege,Beijing.Inplanttissueculture,mediumandapparatusThecompletesterilizationofthedishand

2、thecorrectasepticoperationarethepreventionofdyeingThekeytothenormalgrowthanddifferentiationofbacteriaandtissueculturematerialsisThemostbasicandimportanttechniqueingroupwork.EngagedintheScientificworkersrelatedtogroupworkshouldbeinpracticeCanbeflexibleandcleveraccordingtothenature,conditions

3、andrequirementsoftheworkTheapplicationoftheprinciple,skilled,agile,andcorrectlycarriedoutOperation.However,inteachingandresearch,therearealwayssomeIncorrectoperationreflectsarecognitionoftheconceptsandprinciplesThevaguenessofknowledge.Let'stalkaboutsomeoftheproblemsI'vehadAndviews,forthepee

4、rtolearnfrom.Sterilization,sterilizationandsterilizationThetermsterilizationreferstotheuseofphysicalorchemicalmethodstokillAllmicroorganismsonthesurfaceofdeadobjectsandinside.ItisdifferentfromtheDisinfection.DisinfectionisakindofhealthcarethatcomesfrommedicineShi,usuallyreferstothekillingof

5、harmfulpathogenicmicroorganisms.InadditiontobacteriaisToremoveliquidsorgasesbymechanicalorotherphysicalmeansThemicroorganismsinthemicroorganismachievethepurposeofsterilepurification.VisiblesterilizationTheobjectormediumafterthebacteriaissterileanddisinfectedObjectsmaystillcontainalotofmicro

6、bes.InteachingandworkingItisoftenheardthatsterilizationiscalledsterilization,andthebacteriaissaidtobeSterilization,orsterilization,sterilizationandantibacterialthreedifferentconceptsConfused.Thisshouldbeclarified.HightemperaturesterilizationHightemperaturesterilizationistheuseofheattokillmi

7、croorganisms.TheoriginalItisthehightemperaturethatdenaturationoftheproteinsintheprotoplasmandthelossofenzymesLiving,causingmicrobialdeath.HightemperaturesterilizationistheplanttissuecultureThemostcommonlyusedsterilizationmethods,mainlyhightemperaturestea