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1、动物细胞培养常用技术（Commontechniquesforanimalcellculture）AnimalcellculturetechnologyAnimalcellcultureTheanimalexperimentcenterofZhongshanUniversityPrefaceIn1885Rouxfirsttrytomaketissueculturedinvitro,chickneuralplatematerialis,usingsalineastheculturemedium,andthefirstuseoftheterm
2、tissueculture.In1907Harrisonand1912Carrelbegantotissuecultureasaresearchmethod,fromthecultureofanimalcellsfor.Theapplicationoftissuecultureinmedicalresearch,suchasantiviralvaccine,nowhasgraduallybecomeafinetechnology.Fostermanycelllines,celllinesuccess,especiallyinthetum
3、orcelllinesestablishedforvariousmaterials,suchasHelacelllines(Gey,1952),canbeusedtocarryoutaseriesofresearch,moretopromotethedevelopmentoftissueculturetechnology.Thehotresearchfieldsintissueculture1)cellinternaleventssuchasDNAreplicationandtranscription,proteinsynthesis,
4、energymetabolism;2)cellinternalflow,suchasrunningRNAfromthenucleustothecytoplasm,hormonereceptorcomplextranslocation;3)ecology,suchasnutrition,infection,virusorchemicalmutagenesis,drugeffects;4)theinteractionbetweencellsandcells,suchasembryonicinduction,cellpopulationdyn
5、amics.TheclassificationandthebasicconceptsoftissuecultureClassification:1.tissueculture(TissueCulture)referstotheremovaloftissuefromthebody,inthesimulatedphysiologicalenvironment,sterile,appropriatetemperatureandnutritionconditions,tosurviveandgrowandmaintainitsstructure
6、andfunction.2.cellculture(CellCulture)isasinglecellcultureandcellpopulations.3.organculture(OrganCulture)isthecultivationoftheorganprimordia,organorpartofawholeorgan,tomaintainacertaingrowthandsurvivalandfunctioninvitro.Cellbiologicalcharacteristicsoftissueculture:Thedif
7、ferencebetweenthe1.cellsinvitroandinvivo:whentheartificialconditionsandtheactualsituationisnotexactlythesameinvivowhencellswereculturedinvitro,oncelostneurohumoralregulationandinteractionsofcells,livinginthelackofdynamicbalanceoftheenvironment,changeisinevitable.Themostc
8、ommoncellperformanceis:(Atavism)phenomenon,namelythereversiontolosetheoriginaltissuestructureandcellmor
