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1、Biomaterials32(2011)9374e9382ContentslistsavailableatSciVerseScienceDirectBiomaterialsjournalhomepage:www.elsevier.com/locate/biomaterialsThepromotionofneuritesproutingandoutgrowthofmousehippocampalcellsinculturebygraphenesubstratesa,ca,daa,caaa,cb,cNingLi,XueminZhang,QinS
2、ong,RuigongSu,QiZhang,TaoKong,LiweiLiu,GangJin,a,c,*a,c,*MingliangTang,GuoshengChengaSuzhouInstituteofNano-TechandNano-Bionics,ChineseAcademyofSciences,398RuoshuiRoad,SuzhouIndustrialPark215123,PRChinabInstituteofMechanics,ChineseAcademyofSciences,15BeisihuanWestRoad,Beiji
3、ng,100190,PRChinacGraduateUniversityoftheChineseAcademyofSciences,19AYuquanRoad,Beijing,100049,PRChinadChangchunUniversityofScienceandTechnology,WeixingRoad,Changchun,Jilin,130022,PRChinaarticleinfoabstractArticlehistory:Graphenehasbeendemonstratedinmanybiomedicalapplicati
4、onsanditspotentialsforneuralinter-Received4August2011facing.Emergingconcernsongraphene,asabiomedicalmaterial,areitsbiocompatibilityandhowAccepted19August2011biologicallytargetedtissue/cellsrespondtoit.Relativelyfewstudiesattemptedtoaddresstheinterac-Availableonline8Septemb
5、er2011tionsofgrapheneoritsderivativeswiththetissues/cells,whileveryfewreportsonneuralsystem.Inthisstudy,wetriedtoexplorehowneurites,oneofthekeystructuresforneuralfunctions,areaffectedbyKeywords:grapheneduringthedevelopmentuntilmaturationinamousehippocampalculturemodel.Ther
6、esultsNeuron-favorableCVDgraphenerevealthatgraphenesubstratesexhibitedexcellentbiocompatibility,ascellviabilityandmorphologyGAP-43werenotaffected.Meanwhile,neuritenumbersandaverageneuritelengthongrapheneweresignifi-Neuritesprouting&outgrowthHippocampuscantlyenhancedduring2e
7、7daysaftercellseedingcomparedwithtissueculturepolystyrene(TCPS)substrates.EspeciallyonDay2oftheneuraldevelopmentperiod,graphenesubstratesefficientlypromotedneuritesproutingandoutgrowthtothemaximalextent.Additionally,expressionofgrowth-associateprotein-43(GAP-43)wasexaminedi
8、nbothgrapheneandTCPSgroups.WesternblotanalysisshowedthatGAP-43expressionwasgreatlyenhance
