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1、牛乳铁蛋白单克隆抗体的制备沈阳张英华东北农业大学食品学院，哈尔滨150030摘要：以LF(牛乳铁蛋白)为抗原对BALB/C小鼠进行免疫，利用杂交瘤技术进行融合，制备了3株抗牛LF的杂交瘤细胞，并对其浓度、上清效价、腹水效价、抗体亚类、分泌单克隆抗体稳性定、单克隆抗体分子量等进行鉴定。结果表明，抗体分泌稳定，腹水效价达到106，间接ELISA、Westernblot结果显示3株单抗均与FL发生特异性结合；Ig亚类鉴定均为IgG，且轻链均为K链。牛乳铁蛋白；免疫；鉴定；单克隆抗体S767.5;X17A1005-9369(2011)11-0039-06Prepa
2、rationofmonoclonalantibodyagainstbovinelactoferrinSHENGYangZHANGYinghua2010-12-17国家农业科技成果转化资金项目（子课题）沈阳（1983-），女，硕士研究生，研究方向为牛乳铁蛋白检测试剂盒的研发。E-mail:shenyangshipin@163.com张英华，副教授，研究方向为食品科学。E-mail:yhzhang2000@126.comoralpropertiesofporcineplasmaproteinhydrolysateasinfluencedAnalyticalBi
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