欢迎来到天天文库
浏览记录
ID:53569245
大小:530.34 KB
页数:9页
时间:2020-04-18
《国内外蚕豆核心种质SSR遗传多样性对比及微核心种质构建-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、作物学报ACTAAGRONOMICASINICA2014,4O(7):1311—1319http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E—mail:xbzw@chinajouma1.net.caDoI:10.3724/SP.J.1006.2014.01311乎\∞el;研究简报$(,e/言、、S/、s/;、国内外蚕豆核心种质SSR遗传多样性对比及微核心种质构建姜俊烨杨涛王芳方俐仲伟文2关建平宗绪晓1,中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081;甘肃农业大学生命科学技术学院,
2、甘肃兰州730070摘要:运用24对SSR引物,对国内外1075份初级地理蚕豆核心种质的遗传多样性分析显示,等位变异数、有效等位变异数及Shannon’s信息指数分别为8.54、2.26和1.02;对全部参试资源进行聚类分析,没有发现明显的群体结构,表明初级地理核心种质的代表性较好,遗传背景较广泛。之后采用每个类内随机抽样的方法构建含有129份国内资源和63份国外资源的蚕豆微核心种质,等位基因变异数、有效等位变异数和Shannon’s信息指数的保留比例分别为87.32%、101.26%和101.82%;经t检验得出微核心种质与全部参试资源群体问遗传多样性差异不显著,表明构建的
3、微核心种质的遗传多样性可以代表初级地理蚕豆核心种质。关键词:SSR;蚕豆;遗传多样性;核心种质;微核心种质GeneticDiversityAnalysisofGermplasmResourcesandConstructionofMini-coreCollectionsforViciafabaL.atHomeandAbroadJIANGJun—Ye,YANGTao,WANGFang,FANGLi1,ZHONGWei.Wen,GUANJian—Ping,andZONGXu—XiaO,NationalKeyFacilityforCropGeneResourcesandGenetic
4、Improvement/InstituteofCropScience,ChineseAcademyofAgriculturalSciences,Beijing100081,China;CollegeofLifeScienceandTechnology.GansuAgriculturalUniversity,Lanzhou730070,ChinaAbstract:Thegeneticdiversityof1075genotypesfromaprimarygeographiccorecollectionoffababean(ViciaL.)wasanalyzedbyusingSS
5、Rmarkers.Thenumberofobservedandefectiveallelesof8.54and2.26.theShannon’sinformationindexof1.02.weredetected.Unconspicuouspopulationstructureamongthe1075genotypesindicatedanextensivegeneticbackgroundandnicerepresentativeness.Amini.corecollectioncomprised129Chineseand63overseafababeangenotype
6、swasrandomlysampledfromeachclusterofdendrogramincludingallthe1075testedgenotypes.Theobservedalleles.efectiveallelesandShannon’sinformationindexofthemini.corecollectioncovered87-32%,101.26%。and101.82%oftheprimarygeographiccorecollectionrespectively.Thet-testdidnotshowsignificantdiferencesing
7、eneticdiversitybetweentheprimarygeographiccorecollectionandthemini.corecollection.whichvalidatedtherepresentativenessofthemini—corecollectioningeneticdiversityoffababean.Themini.corecollectionwillplayanimportantroleinexplorationofnewgeneswithinfababean.K
此文档下载收益归作者所有