大豆蛋白水解物促乳酸菌增殖发酵的研究-论文.pdf

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1、生三程Vo1．33，No．16，2012大豆蛋白水解物促乳酸菌增殖发酵的研究白凤翎’，曲玲童’，张柏林，赵宏飞(1．渤海大学化学化工与食品安全学院，辽宁省食品安全重点实验室，“食品贮藏加工及质量安全控制工程技术研究中心”辽宁省高校重大科技平台，辽宁锦州121013；2．北京林业大学生物科学与技术学院，北京100083)摘要：应用小型生物发酵罐对乳酸菌增殖作用进行发酵研究，结果表明，12g／100mL)~脂乳中添加大豆蛋白水解物增殖复合剂对嗜热链球菌和保加利亚乳杆菌生长具有显著的促进作用。添加酵母膏脱脂乳培养基的产酸量比脱脂乳增加l倍．添加增殖复合剂后为脱脂乳的3．12倍。添加增殖复

2、合剂可使乳酸茵数量达到l0个数量级，比脱脂乳培养基提高1—2个数量级。从累积添加碱量和单位加碱量两项指标来看，脱脂乳培养基乳酸菌生长呈现缓慢上升曲线．添加酵母膏呈现三段波浪式曲线，添加增殖复合剂为陡峭的上升曲线。单纯添加酵母膏不能支持乳酸茵连续生长．添加大豆蛋白水解物和酵母膏能够使乳酸菌生长快速启动，并维持一个较高生长水平，获得较大的细胞量的同时也充分利用乳中蛋白质关键词：大豆蛋白水解物，乳酸菌，发酵，增殖StudyonsoyproteinpromotingproliferationfermentionofIacticacidbacteriaBAIFeng-lingI,QULing

3、-tong，ZHANGBo-lin2,ZHAOHong-fei(1．CollegeofChemistry，ChemicalEngineeringandFoodSafety，BohaiUniversity，FoodSafetyKeyLabofLiaoningProvince，EngineeringandTechnologyResearchCenterofFoodPreservation，ProcessingandSafetyControlofLiaoningProvince，Jinzhou，Liaoning121013，China；2．CollegeofBiologicalScien

4、ceandBiotechnology，BeijingForestryUniversity，Beijing100083，China)Abstract：ThefermentationtestsofproliferationonIacticacidbacteria(LAB)wascarriedoutinbioreactor．theresultsshowedthatgrowthofStreptococcusthermophilusandLactobacillusbu

5、Qaricusweresignificantlypromotedin12g／1OOmLskimmilksupplemente

6、dwithsoyproteinhydrolysates(SPH)．TheacidproductionofLABonnonfatmilksupplementedwithyeastextractsandmultiplicationcomplexwere2and3．12timesthanthatofcontro1．ThenthecellcountofLABreachedto101gcycleonskimmilksupplementedwithmultiplicationcomplex．whichincreased1．0～2．01gcyclethancontroI．Theparameter

7、ofaccumulateadditionaIalkalivolumeandintervaladditionalalkal}volumeshowedthatthegrowthcurveofLABonskimmllkascendslowly．andthreewavestrendassupplementedwithyeastextract，andwentupdramaticallywithadditionofmultip¨cationcomplex．TheadditionofyeastextractalonecannotsupportthecontinualgrowthofLAB．how

8、ever．theadditionofyeastextractandproteinhydrolytestogethercanaccelerateitandmadeittokeepinahighgrowthlevelMuchmorebiomassofLABonmilkwithmultiplicationcomplexwouldbeobtainedandtheproteinjnmilkwouldbeutilizedsufficiently．Keywords：soyprote