象皮生肌散对实验大鼠慢性皮肤溃疡表皮生长因子表达影响的研究

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1、中国中西医结合皮肤性病学杂志2010年第9卷第4期·213··论著·象皮生肌散对实验大鼠慢性皮肤溃疡表皮生长因子表达影响的研究席建元，贺春辉，左和宁，胡金辉，荣光辉。，贺菊乔(1．湖南中医药大学第一附属医院，长沙410007；2．湖南耒阳市人民医院，耒阳421800；3．湖南中医药大学，长沙410007)摘要：目的观察象皮生肌散对慢性皮肤溃疡大鼠创面新生肉芽组织中表皮生长因子(EGF)水平的影响，探讨象皮生肌散对皮肤溃疡愈合的作用机理。方法将90只SD大鼠随机分成象皮生肌散组、康复新液组和模型组，每组3O只，参照沈道修改良塑料环

2、肉芽肿定量法，经改进制成慢性皮肤溃疡模型，组内再随机分成术后5d、10d2个时间点，每个时间点处死10只大鼠，术前禁食，用SABC免疫组化法检测大鼠创面新生肉芽组织中EGF的表达。结果象皮生肌散组平均愈合时间较康复新液组及模型组分别提前4d(P<0．05)及7d(P<0．01)；象皮生肌散组局部创面新生肉芽组织中EGF的计数与分布都优于模型组(P<0．01)。结论象皮生肌散能够增加皮肤溃疡创面肉芽组织中EGF的表达，对皮肤溃疡组织的愈合有促进作用。关键词：象皮生肌散；大鼠；慢性皮肤溃疡；表皮生长因子中图分类号：R285，R753

3、．7文献标识码：A文章编号：1672—0709(2010)04—0213—03EfectofXiangpiShengjiPowderontheExpressionofEpidermalGrowthFactoronChronicSkinUlcerofRatXIJian-yuani，HEChun—hui2,ZUOHe—nin，HUJin—huil，RONGGuang-hui3,HEJu—qiaoI．TheFirstAfiliatedHospitalofHunanUniversityofTraditionalChineseMedici

4、ne．Changsha41OOOZChinc~ZPeople’SHospitalofLeiyangCity,Leiyang421800,China,．3．HunanUniversityofTraditionalChineseMedwine，Changsha410007,ChinaAbstract：ObjectiveToobservetheeffectsofXiangpiShengjipowderontheexpressionofepidermalgrowthfactor(EGF)onthenewborngranulationof

5、chronicskinulcerofratmodels，andtodiscussthefunctionofXiangpiShengjipowderinpromtingwoundhealing．Methods90SDratswererandomlydividedintoXiangpiShengjipowdergroup，KangfuxinYegroupandmodelgroupwith30ratsineachgroup．Accordingtotheamelioratingplasticringquantifyingmethodof

6、ShenDaoxiu，theratsweremadeintochronicskinulcermodels．Eachgroupwasrandomlydividedintotwotimepointsofthe5thdaysand10thdaysafteroperation．10ratswerekilledineachgroupeverytimeafterabrosia，andtheexpressionofEGFwasdetectedbyimmunohistochemicalmethod．ResIlltsTheaverageheali

7、ngtimeofXiangpiShengjipowdergroupwas4days(尸l<0．05)earlierthanthatofKangfuXinliquidgroupand7days(Pl<0．O1)earlierthanthatofmodelgrouprespectively．ThecountanddistributionoftheEGFinthenewborngranulationofXiangpiShengjipowdergroupweresuperiortothatofmodelgroup(P<0．01)．Con

8、clusionXiangpiShengjipowdercanincreasetheexpressionofEGFinthegranulationtissuleofwoundSOastopromotewoundhealing．Keywords：XiangpiShe