羊膜载体复合膜负载骨髓间充质干细胞促愈创面的研究

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1、202qActaAcadMedWeifangVof．32No．32010doi：10．3969／j．issn．1004-3101．2010．03．015羊膜载体复合膜负载骨髓间充质干细胞促愈创面的研究王冰，钟伟，刘儒森(潍坊医学院外科学教研室，山东潍坊261053；潍坊医学院附属医院骨科)AStudyonBoneMarrowMesenchymalStemCellsLoadedbyCattleAmnionGelatinChitosanRepairingSkinWoundDefectWANGBing’ZHONGWei，LIURu．sen，(Surgic

2、alDepartmentofWeifangMedicalCollege，Weifang261053，China；。OrthopedicsDepartment，fAffiliatedHospitalofWeifangMedicalCollege)[ABSTRACT]ObjectiveToinvestigatetheabilityandeffectofbonema~owmesenchymalstemcells(MSCs)onenhancingthehealingofexperimentalepidermaldefect，toprovideanewsou

3、rceoforiginalcellsforskinwoundre-pair．MethodsTheMSCsofrabbitswereculturedandpurifiedinvitro，thenlabeledwith5一bromodeoxy—uridine(Br—dU)．Fullskinlosswoundwasestablishedonbothsidesofthebackofrabbitwhosebonemarrowshadbeenobtained．ThelabeledMSCsusingcattlegelatinchitosanasthevector

4、wereregrafledbacktodonoranimalgwoundintheleftside(treatmentgroup)．Thewoundintherightsidewasgivencattleamniongelatinchitosanonly(controlgroup)．Thewoundcontractionratewasobservedafter1，2weeksandthehealingtimewasrecorded．Theregenerationtissuesinthecenterofthewoundwereharvested4to

5、5weeksaftertheoperationandwerestainedbyBrdU．ResultsTherewassignificantdifferencebetweenthetwogroups(P<0．05)incontractionrateandhealingtimeofthewound．Theepidermiswasthic—kerintreatmentgroupthanthatincontrolgroup．AfewBrdUpositivecellswereappearedinthehairfollicleofthere—generati

6、onskinandbasallayerofepidermis．ConclusionMSCsautografiingmayimproveconsiderablythequalityofwoundhealingtosomedegree。whichmayopenanew—waytoreachinga“perfectrepair’’fromanatomicalrecoverytofunctionalrecoveryafterskininjury．[KEYWORDS]Bonema~owmesenchymalstemcells；Woundhealing；Ski

7、n[作者简介]王冰(1983年～)，男(汉族)，山东省潍坊市人，在读硕士研究生。主要研究方向：骨创伤外科学。[通讯作者]钟伟，E-mail：zhw5687@yahoo．eom．cn．．潍坊医学院学报2010年第32卷第王期203如何使受创组织达到完美愈合是当前组织修复中白酶恒温水浴振荡器内消化3h，PBS洗3次。的重要问题。随着组织工程化研究的拓展，应用干细1．3动物移植实验胞技术有望解决这一难题。骨髓间充质干细胞(mes—1．3．1动物模型制备兔背部脊柱两侧，各制造半径enchymalstemcells，MSCs)是一种具有多向分化潜能为2cm的

8、圆形全层皮肤缺损创面，以羊膜复合膜为载的细胞，来源丰富，分离培养容易，已成为组织工程研体，将BrdU标记的自体MSCs涂布