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1、中国组织工程研究与临床康复第14卷第5期2010–01–29出版JournalofClinicalRehabilitativeTissueEngineeringResearchJanuary29,2010Vol.14,No.5Surgicaltechniquesoforthotopiclivertransplantationinratsbyasingleoperatorunderdirectvision☆12LinYong-hua,JiangYiAbstract1FuzongClinicalBACKGROU
2、ND:RatmodeloforthotopiclivertransplantationisaveryvaluablemodelforexperimentalstudyinliverCollegeofFujiantransplantationincludingorganpreservation,tissueischemia-reperfusioninjury,allograftrejectionandimmunetoleranceMedicalUniversity,mechanism.Stablelivert
3、ransplantationanimalmodelisthebasisoftherelatedexperimentalstudies.However,itsFuzhou350025,FujianProvince,experimentaloperationislongandboring,especiallyperformedbyasingleoperatorunderdirectvision.2China;DepartmentOBJECTIVE:Toinvestigatetheoperationtechniq
4、uestoestablishastableratmodeloforthotopiclivertransplantationbyaofHepatobiliarysingleoperatorunderdirectvision.Surgery,FuzhouMETHODS:Theorthotopiclivertransplantationwasperformedusingtwo-cuffmethodin50pairsofrats.WeexposedtheGeneralHospitalofNanjingMilitar
5、yAreaabdominalcavityfully,perfusedthedonorliverthroughabdominalaortawithoutflippingdonorliver;suprahepaticinferiorvenaCommandofcavawasinvivocutdownusingone-stepmethod,withoutdiaphragmring;thesuprahepaticinferiorvenacavawasChinesePLA,anastomosedwithsingle-r
6、owsuture,andthecuffofportalveinwasinstalledbyfixingthebloodvesselforcepsonrubber.Fuzhou350025,Hepaticarterywasnotreconstructed.FluidreplacementwasadministeredtomaintainhemodynamicstabilityinratsafterFujianProvince,Chinaoperation.RESULTSANDCONCLUSION:Thedon
7、oroperativetimewas(36.2±2.5)minutes,donorlivertrimmingtimewas(12.2±1.5)LinYong-hua☆,minutes,receptoroperativetimewas(45.6±3.5)minutes,suprahepaticinferiorvenacavaanastomosistimewas(10.1±2.1)Studyingforminutes,portalveincufftimewas(1.5±0.9)minutes,infrahepa
8、ticinferiorvenacavecufftimewas(1.1±0.6)minutes,doctorate,Attendingphysician,Fuzonganhepaticphasewas(15.1±2.2)minutes.Thesuccessratiooftheoperationwas100%andthesurvivalrateswithin1weekClinicalCollegeofand1mont
