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时间:2020-04-17
《Krupple样转录因子6和核组蛋白-2在大鼠胃癌模型胃肠道组织中的表达.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、·1944·2o14年9月第31卷第9期ChinJExpSurg,September2014,Vo1.31o.9·实验研究·Kriipple样转录因子6和核组蛋白一2在大鼠胃癌模型胃肠道组织中的表达王建锋王耿泽宋展厉冰【摘要】目的观察在胃癌大鼠模型胃肠道组织中Kriipple样转录因子6(Klf-6)和核组蛋白一2(NUCB2)及相关基因表达的影响。方法将Wister大鼠96只分为3组,A组32只,采用黄曲霉素B1(AFB1)对大鼠进行诱导处理3个月,制备胃癌大鼠模型。B组32只,应用AFB1对大鼠进行诱导处理6个月,制备胃癌大鼠模型。C组32只,为正常
2、对照组。用免疫组织化学法检测3组大鼠胃肠道组织中NUCB2和Klf-6的表达水平。逆转录一聚合酶链反应(RT—PCR)技术检测两组大鼠胃肠道组织中NUCB2和Klf-6基因mRNA表达水平,比较其差异。结果A、B组成功制备了胃癌大鼠模型,但不同诱导时间两组大鼠胃癌发病的程度和发病率不同,差异有统计学意义(P<0.05)。Klf-6和NUCB2在B组的表达率高于A组和C组,差异有统计学意义,A组K1和NUCB2表达又高于C组,差异有统计学意义(.=r=3.17、4.95、3.79,P<0.05)。RT—PCR结果显示,B组胃肠道组织中Klf-6和NUCB2
3、基因的mRNA表达水平高于A组,A组高于c组,差异有统计学意义(X2=2.98、4.13、3.52,P<0.05)。结论肌氨酸乙酯盐酸盐诱导胃癌模型时间越长,胃癌大鼠胃肠道Klf-6和NUCB2的表达也越强。【关键词】胃癌;Krapple样转录因子6;核组蛋白-2ExpressionofKriippel-likefactor6andnucleobindin-2genesingastrointestinaltractofratswithgastriccancerWangJianfeng,WangGengze,SongZhan,LiBing.Departme
4、ntofGeneralSurgery,NanyangCentralHospitaZ.Nanyang473000,ChinaCorrespondingauthor:WangJianfeng,Email:Jianfeng2890@163.corn【Abstract】ObjectiveTodiscusstheexpressionofKrtippel—likefactor6(Klf-6)andnucleo·bindin一2(NUCB2)genesingastrointestinaltractofratswithgastriccancer.MethodsNinet
5、y—sixadultWisterratswererandomlydividedintothreegroups:32ratseachingroupsAandBweredrenchedintraper—itoneallywithsarcosineethylesterhydrochloridetoinducegastriccancerfor3and6monthsrespectively,and32ratsingroupCwereinjectedwithnormalsaline.Thegastrointestinaltractwasexaminedbyelect
6、ronicscaleat3rdand6thmonthaftermodeling.rheI(1fandNUCB2expressioningastrointestinaltractofratswasobserved,andthemRNAlevelswereevaluatedbyreversetranscription—polymerasechainreaction(RT—PCR)aftertheratsweredecapitated.ThestatisticalanalysiswasdonebyusingSPSS14.0statisticalsoftware
7、.ResultsA11the96ratswereinvolvedintheanalysisofresults.PositiverateofK1f-6andNUCB2ingroupAwashigherthaningroupsBandC,andthatingroupBhigherthaningroupC(X2=3.17,4.95,and3.79,P<0.05).RT—PCRrevealedthattheexpressionlevelsofKlf-6andNUCB2mRNAingroupAwerehigherthaningroupsBandC,andthose
8、ingroupBhigherthaningroupC(=2.98,4.13and
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