临床医学毕业论文非人工通气下大鼠急性心肌梗死模型的建立.doc

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1、XX大学毕业论文非人工通气下大鼠急性心肌梗死模型的建立2014年6月25日非人工通气下大鼠急性心肌梗死模型的建立作者：魏峰，张静，韩克，刘昱，王亭忠，倪雅娟，马爱群【摘要】FI的在非人工通气条件下，以较小的创伤且简单、快速地建立大鼠急性心肌梗死模型。方法30只成年SD大鼠麻醉后，在自主呼吸条件下，于胸骨左缘第4肋间钝性分离肋间肌，暴露心脏，迅速结扎左冠状动脉前降支(LAD)建立心肌梗死模型。同吋记录术前、术屮及术后1、3d,1、2周的心电图变化;术后2周开胸，观察心脏大体形态的改变，LAD供血区出现明显发白的梗死区即为模型建立成功;同吋取8只模型的心脏标木行组织病理学HE染色以证实模型建立

2、。结果25只大鼠成功建立心肌梗死模型，模型存活率为83.33%。心电图监测显示LAD结扎后出现R波峰减低，随即出现ST段抬高和ST-T融合，30min后可见病理性Q波。术后2周开胸，可见缺血区心肌颜色发白，室壁运动减弱。HE染色可见心肌梗死区心肌细胞消失，代之大量纤维和胶原组织增生，梗死周边区残存心肌排列紊乱、肌丝断裂，间质结构破坏、纤维组织增生。结论本方法可在非人工通气条件下，快速、简单、有效地建立大鼠急性心肌梗死模型。【关键词】非人工通气;心肌梗死;动物模型;大鼠ABSTRACT:ObjectiveToestablishamodelofacutemyocardialinfarction

3、inratswithoutartificialvcntilation.McthodsAftcranesthesia,leftthoracotomywasperformedin30adultSDratsbybluntdissectionofintercostalmusclesofthefourthintercostalspace,andtheleftanteriordescending(LAD)coronaryarterywasligatedtoestablishamodelofmyocardialinfarctionunderspontaneousbreathingcondition.Ch

4、angesofelectrocardiographywererecordedduringtheoperationand1day,3days,1weekand2weeksaftermodelestablishment.Histomorphologicalchangesoftheheartwereobservedunderthoracotomy2weeksafteroperation.Paleappearanceinischemicmyocardiumwouldsuggestthesuccessfulestablishmentofmyocardialinfarctionandeightmode

5、lswerekilledtodeterminepathologicalchangesofmyocardialinfarctionbyHEstaining.ResultsTwenty-fivemodelsofmyocardialinfarctionweresuccessfullyestablishedwithasurvivalrateof83.33%2weeksafteroperation.AfterligationofLAD,theSTsegmentelevatedandthepathologicalQwaveappearedafter30min.Twoweeksafterestablis

6、hmentofmyocardialinfarctionmodel,ischemicmyocardiumshowedpaleappearanceandthemotionofventricularwallbecameweaker.HEstainingrevealedthatcardiomyocytesdisappearedandwerereplacedbyfibroustissuesandcollagen.Remnantcardiomyocyteswerearrangeddisorderlyandmyofiberswerefractured,withinterstitialdamageandf

7、ibroustissuehypcrplasia.ConclusionBythismethod,amodelofacutemyocardialinfarctioninratscanbeestablishedsimplyandeffectivelywithoutusingartificialventilation.KEYWORDS:non-artificialventilation;myocardialinfarction;