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时间:2020-04-16
《抗草甘膦转基因大豆对雄鼠睾丸的影响-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、第35卷第3期暨南大学学报(自然科学与医学版)Vo1.35NO.32014年6月JournalofJinanUniversity(NaturalScience&MedicineEdition)Jun.2014抗草甘膦转基因大豆对雄鼠睾丸的影响芦春斌,蔡娟,戚青林,隗洋洋(暨南大学生命科学技术学院发育与再生生物学系,广东广州510632)[摘要]以雄性昆明小鼠为动物模型,分别以抗草甘膦转基因大豆和非转基因大豆饲料喂食,于30、90d后,取其睾丸.采用流式细胞术检测睾丸组织中各生殖周期细胞比例、Hoeehst33258染色观察睾丸细胞凋亡、组织化学染色技术观察睾丸组织病理学变
2、化.研究抗草甘膦转基因大豆对雄鼠睾丸的影响.结果显示,30、90d实验组和对照组小鼠睾丸精细胞单倍体、2倍体、4倍体比例均正常且趋于稳定,单倍体比例占主导地位,且实验组与对照组相比无统计学差异.在荧光显微镜下实验组睾丸细胞的细胞核呈正常的蓝色,与对照组相比,未发现明显的细胞核呈致密浓染,或呈碎块状致密浓染,颜色有些发白的凋亡细胞.组织病理观察也未发现两组小鼠的睾丸出现明显病理损伤.30d喂食抗草甘膦转基因大豆急性毒理研究和90d喂食抗草甘膦转基因大豆亚慢性毒理研究对雄鼠睾丸均无显著影响.[关键词]转基因大豆;雄鼠;流式细胞术;细胞凋亡;组织病理[中图分类号]Q788[文献
3、标志码]A[文章编号]t000—9965(2014)03—0245—05Effectsofglyphosate-resistanttransgenicsoybeanonmalemicetestisLUChunbin,CAIJuan,QIQinglin,WEIYangyang(DepartmentofDevelopmentalandRegenerativeBiology,CollegeofLifeScienceandTechnology,JinanUniversity,Guangzhou510632,China)[Abstract]MaleKunmingmicemodels
4、exposuretoglyphosate—resistanttransgenicsoybeanandNon—GMOsoybeanwereestablishedandusedtoevaluatetheimpactsofglyphosate-resistanttransgenicsoybeanO13.thetestis.Themicewerefedfor30and90daywithglyphosate—resistanttransgenicsoybeanandNon—GMOsoybean,respectively,andthenthemicetesteswerecollect
5、ed.Changesoftesticulartissuecellre—productivecyclesweredetectedbyflowcytometry,andtesticulartissuecellapoptosiswereassessedbyHoechst33258dye.Thepathologicalchangesofmicetesteswereexaminedbyparaffinsections.There—suhsshowedthatthepercentagesoftestiscellpopulations(haploid,diploid,tetraploi
6、d)wasnodifferencebetweentheexperimentalgroupandcontrolgroup(P>0.05),andtheapoptosisofwhitetesticularcell,nuclearthickdenseorfragmentalthickdensewasnotobserved.Thepathologicalchangesoftesticulartis—sueswerealsonotobservedinparaffinsections.Therewasnosignificantdifferenceinmalemicetesticu—l
7、artissuechangesbetweentransgenicsoybeanandNon—GMOsoybean.Theacutetoxicologyresearchby30dayfeedingglyphosate-resistanttransgenicsoybeanandthechronictoxicologyresearchby90dayfeed-·ingglyphosate·resistanttransgenicsoybeanswerenodifference.[Keywords]transgenicsoybean;mi
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