抗草甘膦转基因大豆对雄鼠睾丸的影响-论文.pdf

《抗草甘膦转基因大豆对雄鼠睾丸的影响-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、第35卷第3期暨南大学学报(自然科学与医学版)Vo1．35NO．32014年6月JournalofJinanUniversity(NaturalScience＆MedicineEdition)Jun．2014抗草甘膦转基因大豆对雄鼠睾丸的影响芦春斌，蔡娟，戚青林，隗洋洋(暨南大学生命科学技术学院发育与再生生物学系，广东广州510632)[摘要]以雄性昆明小鼠为动物模型，分别以抗草甘膦转基因大豆和非转基因大豆饲料喂食，于30、90d后，取其睾丸．采用流式细胞术检测睾丸组织中各生殖周期细胞比例、Hoeehst33258染色观察睾丸细胞凋亡、组织化学染色技术观察睾丸组织病理学变

2、化．研究抗草甘膦转基因大豆对雄鼠睾丸的影响．结果显示，30、90d实验组和对照组小鼠睾丸精细胞单倍体、2倍体、4倍体比例均正常且趋于稳定，单倍体比例占主导地位，且实验组与对照组相比无统计学差异．在荧光显微镜下实验组睾丸细胞的细胞核呈正常的蓝色，与对照组相比，未发现明显的细胞核呈致密浓染，或呈碎块状致密浓染，颜色有些发白的凋亡细胞．组织病理观察也未发现两组小鼠的睾丸出现明显病理损伤．30d喂食抗草甘膦转基因大豆急性毒理研究和90d喂食抗草甘膦转基因大豆亚慢性毒理研究对雄鼠睾丸均无显著影响．[关键词]转基因大豆；雄鼠；流式细胞术；细胞凋亡；组织病理[中图分类号]Q788[文献

3、标志码]A[文章编号]t000—9965(2014)03—0245—05Effectsofglyphosate-resistanttransgenicsoybeanonmalemicetestisLUChunbin，CAIJuan，QIQinglin，WEIYangyang(DepartmentofDevelopmentalandRegenerativeBiology，CollegeofLifeScienceandTechnology，JinanUniversity，Guangzhou510632，China)[Abstract]MaleKunmingmicemodels

4、exposuretoglyphosate—resistanttransgenicsoybeanandNon—GMOsoybeanwereestablishedandusedtoevaluatetheimpactsofglyphosate-resistanttransgenicsoybeanO13．thetestis．Themicewerefedfor30and90daywithglyphosate—resistanttransgenicsoybeanandNon—GMOsoybean，respectively，andthenthemicetesteswerecollect

5、ed．Changesoftesticulartissuecellre—productivecyclesweredetectedbyflowcytometry，andtesticulartissuecellapoptosiswereassessedbyHoechst33258dye．Thepathologicalchangesofmicetesteswereexaminedbyparaffinsections．There—suhsshowedthatthepercentagesoftestiscellpopulations(haploid，diploid，tetraploi

6、d)wasnodifferencebetweentheexperimentalgroupandcontrolgroup(P>0．05)，andtheapoptosisofwhitetesticularcell，nuclearthickdenseorfragmentalthickdensewasnotobserved．Thepathologicalchangesoftesticulartis—sueswerealsonotobservedinparaffinsections．Therewasnosignificantdifferenceinmalemicetesticu—l

7、artissuechangesbetweentransgenicsoybeanandNon—GMOsoybean．Theacutetoxicologyresearchby30dayfeedingglyphosate-resistanttransgenicsoybeanandthechronictoxicologyresearchby90dayfeed-·ingglyphosate·resistanttransgenicsoybeanswerenodifference．[Keywords]transgenicsoybean；mi