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时间:2020-04-16
《绞股蓝总皂苷脂质体的制备与表征-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、·2526·中革1I5ChineseTraditionalandHerbalDrugs第44卷第18期201三堡旦绞股蓝总皂苷脂质体的制备与表征喻樊,。1.盐城师范学院药学院,江苏盐城22405l2.盐城师范学院江苏省滩涂生物资源与环境保护重点建设实验室,江苏盐城224002摘要:目的探讨用豆甾醇替代胆固醇制备绞股蓝总皂苷脂质体的可行性,并获得最佳制备工艺与处方。方法以卵磷脂和豆甾醇为膜材制备不含胆固醇的绞股蓝总皂苷脂质体;脂质体以鱼精蛋白沉淀法进行包封率的测定;以包封率为评定指标,筛选该新型绞股蓝总皂苷脂质体的最佳制备方法;采用正交设计优化该脂质体的制备工艺;采用粒径、Zeta电位、
2、原子力显微镜(AFM)表征该新型绞股蓝总皂苷脂质体。结果用豆甾醇替代胆固醇制备绞股蓝总皂苷脂质体的最佳制备方法为乙醇注入法,通过正交设计得最佳工艺为绞股蓝总皂苷与卵磷脂的质量比为1:10,卵磷脂与豆甾醇的质量比为4:1,PBS缓冲溶液pH值为7.0,水化温度为35℃;所得脂质体的包封率为(77.00±1.17)%,粒径为(202.6±3.9)nlTl,多分散系数(PDI)为0.108±0.003,电位为(一28.66±0.86)mV,该脂质体在电镜下呈规则圆形。结论用豆甾醇替代胆固醇制备绞股蓝总皂苷脂质体方法可行,采用最佳工艺制备的绞股蓝总皂苷包封率高,形态和粒径均较好,重现性好。关键
3、词:绞股蓝总皂苷;脂质体;豆甾醇;制备工艺;正交设计中图分类号:R283.6文献标志码:A文章编号:0253—2670(2013)18-2526—05D0I:10.7501/j.issn.0253.2670.2013.18.007PreparationandcharacterizationofgypenosidesliposomesconsistedbylecithinandstigmasterolYUFan,1.CollegeofPharmacy,YanchengTeachersUniversicy,Yancheng224051,China2.JiangsuProvincialKey
4、LaboratoryofCoastalWetlandBioresourcesandEnvironmentalProtection,YanchengTeachersUniversity,Yancheng224002,ChinaAbstract:ObjectiveTostudythefeasibilityofpreparinggypenosideliposomes(GLs)withstigmasterolinsteadofcholesterol,andtooptimizetheprescriptionandpreparationofGLs.MethodsGLswithoutcholeste
5、rolwerepreparedbyethanolinjectionmethodwithlecithinandstigmasterol,andtheencapsulationeficiencywasdeterminedbyprotamineprecipitationmethod.Theencapsulationeficiencywasadoptedtoevaluateandoptimizethepreparationprocessasresponseinorthogonaldesignassay.GLswerecharacterizedbyDynamicLightScatteringan
6、dAtomicForceMicroscope(AFM)usingparticlesizeandelectricpotentialasindexes.ResultsEthanolinjectionwasthebestmethodtoprepareGLswithlecithinandstigmastero1.Theoptimalpreparationprocessdeterminedbyo~hogonaldesignassaywasasfollows:theratioofgypenosidestolecithinwas1:10,theratiooflecithintostigmastero
7、lwas4:1,thepHvalueofPBSbufersolutionwas7.0,andthehydrationtemperaturewas35℃.Theentrapmenteficiencywas(77.004-1.17)%,theparticlesizewas(202.6士3.9)nm,thePD1was0.108-4-0.003,thezetapotentialwas(一28.66土0.86)mV,andthemorphologywa
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