RT-PCR检测卵巢肿瘤中EGFR和CK-20的表达及意义.doc

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1、RT-PCR检测卵巢肿瘤中EGFR和CK-20的表达及意义作者：邹忠香，徐杰，沙秀敏，张素华单位：山东，烟台市莱阳中心医院妇产科【摘要】目的研究表皮生长因子受体(epidermalgrowthfactorreceptor,EGFR)基因和细胞角质蛋白20(cytokeratin-20,CK20)在卵巢上皮性肿瘤中的表达及意义。方法应用RT-PCR法检测12例正常卵巢(对照组)、18例卵巢良性肿瘤(良性组)、33例卵巢上皮癌(恶性组)组织屮EGFR、CK20niRNA的表达，分析二者与卵巢肿瘤发生发展及转移的关系。结果(1)原发性卵巢上皮癌组织中

2、EGFR和CK20mRNA的阳性表达率显著高于正常卵巢及卵巢良性肿瘤组织(P<0.05);(2)EGFRmRNA表达与原发性卵巢上皮癌手术病理分期有关，其在III〜IV期卵巢癌患者组织屮的表达阳性率高于I〜II期患者组织(P<0.05);CK20mRNA表达与卵巢上皮癌手术病理分期、淋巴结转移有关(P<0.05),其在III〜IV期和有淋巴结转移组的患者组织中阳性表达率分别高于I〜II期和无淋巴结转移组患者(P<O.05);(3)EGFR与CK20基因表达之间有显著相关性(P<O.05)o结论⑴EGFRmRNA和C

3、K20mRNA在卵巢癌组织中有较高的表达，可能与卵巢癌发生、发展和转移有关；(2)检测卵巢癌组织中EGFRmRNA、CK20mRNA的阳性表达对判断卵巢癌患者的转移和估计预后有一定的参考价值。【关键词】卵巢肿瘤;表皮生长因子受体;细胞角质蛋白20;逆转录聚合酶链式反应ExpressionandsignificanceofEGFRandCK-20inovariancarcinomaZOUZhong-xiang,XUJie,SHAXiu-min,etal.DepartmentofObstetricsandGynecology,ShangdongPr

4、ovinceYantaiCityLaiyangCentralHospital,Yantai265200,China[Abstract]ObjectiveToinvestigatetheexpressionandsignificanceofepidermalgrowthfactorreceptor(EGFR)andcytokeratin-20(CK20)inovarianepithelialtumor,andtoexploretheirrolesinthetumorigenesis,developmentandmetastasisofovaria

5、ntumor・MethodsTheexpressionofEGFRandCK20weredetectedbyreversetranscriptionpolymerasechainreaction(RT-PCR)in12casesofnormalovarytissue,18casesofbenignovariantumorand33casesofprimaryovariancarcinoma.Therelationshipbetweenthetwogenesandthedifferentclinicopathologicalfeatureswer

6、eanalyzed.Results(l)ThepositiveratesofEGFRmRNAandCK20mRNAweresignificantlyhigherinmalignantovarianspecimensthaninthenormalandbenignones(P<0.05).(2)TheexpressionofEGFRmRNAwascorrelatedtoclinicalstageinprimaryovariancarcinoma,EGFRmRNAinthepatientswithstageIII〜TVhadhigherpos

7、itiveexpressionratethanthatinstageI~II(P<0.05).TheexpressionofCK20mRNAinovariancarcinomawasrelatedwiththeclinicalstageandlymphmetastasis(P&It;0.05).CK20mRNAexpressionratesinpatientswithstageIII~IVandinthegroupwithlymphnodemetastasisweresignificantlyhigherthanthoseinstageI

8、〜IIandinthegroupwithoutlymphnodemetastasisrespectively(P<0.05).(3)Therew