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1、JournalofChinesePharmaceuticalSciences2001,10(3)115MicrobiologicalTransformationofGinsenosideRg1DongAling,CuiYajun,GuoHongzhu,ZhengJunhuaandGuoDean*SchoolofPharmaceuticalSciences,PekingUniversity,Beijing100083ReceivedMay16,2001;AcceptedJuly9,2001AbsractForty-ninemicrobialstrainswereusedtoscree
2、ntheirabilityforthemicrobiologicaltransforma-tionofginsenosideRg1.Aspergillusniger(3.1858)andAbsidiacoerulea(3.3538)werefoundtoconvertginsenosideRg1efficientlytolesspolarmetabolites.PreparativescaletransformationwithbothfungiAbsidiacoerulea(3.3538)andAspergillusniger(3.1858)haveresultedinthepr
3、oductionofonesamemetabolite(MT1).Itsstructurewaschar-1acterizedas6-O-b-D-glucopyranosyl-20(S)-protopanaxatriol(GinsenosideRh1)onthebasisofitsTOF-MSandH,13CNMRspectraldata.ThebiotransformationkineticcurvesforGinsenosideRg1andMT1werereportedforthefirsttime,andthebiotransformationpathwaywaspropos
4、ed.KeywordsMicrobiologicaltransformation;GinsenosideRg1;GinsenosideRh1;Microorganism;FungiIntroductionwerefoundtoconvertginsenosideRg1efficientlyintolesspolarmetabolite.FromtheTLCanalyses,thetrans-Biotransformationisdefinedastheuseofbiologicalformationproductsfromthetwoculturespossessedthesyst
5、emstobringaboutchangesofchemicalstructureinsameRfvalue,whichindicatedthattheycouldbethecompoundsthatarenottheirnaturalsubstances.Thesameproduct.biologicalsystemscouldbeplant,animal,microorgan-ismsorenzymesderivedfromtheorganisms.ManyOptimalconditionofpreparativescaleincubationbiotransformation
6、reactionsarenotonlyregiospecificofginsenosideRg1andstereospecific,butalsoenantiospecificallowingtheTheOrthodoxtestwasdesignedtoobservetheeffectproductionofchiralproductsfromracemicmixtures.offourkeyfactorsonthemicrobiologicaltransformationExistingineverywhere,microorganismsareinfiniteratesucha
7、sculturetemperature,substratequantity,time-1resources.Biotransformationsbymicroorganismshaveofaddingsubstrateandagitationspeed(r×min).Themanyadvantagessuchaslowcost,highyield,mildcon-resultsshowedthatmicrobiologicaltransformationrate[1]
