表达载体pETM-30图谱及序列信息.pdf

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1、EMBLProteinExpressionandPurificationFacilitypETM-30T7promoter-->LacoperatorXbaICGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTGCTTTAATTATGCTGAGTGATATCCCCTTAACACTCGCCTATTGTTAAGGGGAGATCTTTATTAAArbsHis-tagTGTTTAACTTTAAGAAGGAGATATACCATGAA

2、ACATCACCATCACCATCACAACACTAGTAGCAATACAAATTGAAATTCTTCCTCTATATGGTACTTTGTAGTGGTAGTGGTAGTGTTGTGATCATCGTTAMETLysHisHisHisHisHisHisAsnThrSerSerAsnTTCATGTCC..633bp..GACCATCCTCCAACTAGTGGATCTGGTGGTGGTGGCGGATCCATGAGCAAGTACAGG...GST...CTGGTAGGAGGTTGATCACCTAGACCACCA

3、CCACCGCCTAGGTACTCGPheMetSer..211aa..AspHisProProThrSerGlySerGlyGlyGlyGlyGlySerMetSerTEV-siteNcoIGAGAATCTTTATTTTCAGGGCGCCATGGCTGGCAAGCACACA..279bp..GCCGTGTCTATGACACTCTTAGAAATAAAAGTCCCGCGGTACCGACCGTTCGTGTGT...DCOH..CGGCACAGATACTGTGluAsnLeuTyrPheGln

4、GlyAla

5、METAlaGlyLysHisThr...93aa..AlaValSerMetThrNotIBamHISacIEagIKpnIEcoRISalIHindIIIXhoIC-His-tagTAGGTACCGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACATCCATGGCCTAGGCTTAAGCTCGAGGCAGCTGTTCGAACGCCGGCGTGAGCTCGTGGTGGTGGTG***SingleCuttersListedbySiteO

6、rder80EspI190SstI834AsuII3182MstI80Bpu1102I192EcoRI834Csp45I3946Tth111I158XhoI198BamHI1312XbaI4374BsiI160SciI204Acc65I1378BglII4749Eco57I166NotI204Asp718I1419SgrAI5060NruI166XmaIII208KpnI1575SphI5060SpoI166Eco52I313Bsp1407I1784ApaBI5094ClaI166EagI360Pst

7、I2100MluI5275XmaI173HindIII454BspMI2281BstEII5277SmaI179SalI520NcoI2311ApaI5403XorII188EcoICRI660ScaI2511BssHII5403PvuI188Ecl136II662RleAI2550EcoRV6104DraIII190SacI806SwaI2606HpaINonCuttingEnzymesAatIIAflIIAgeIAscIAvrIIBsu36ICspICvnIEam1105IEco31IEco72I

8、FseII-PpoIMfeIMlu113IMstIINdeINheIPacIPinAIPmaCIPmeISacIISauISfiISnaBISplISrfISstIIStuISunILOCUSpETM-306346bpDNAcircular21-NOV-2006SOURCEORGANISMCOMMENTThisfileiscreatedbyVectorNTIhttp://www.invitrogen.com/COMMENTORIGDB

9、GenBankCOMMENTVNTDBDATE

10、427642097

11、

12、COMMENTLSOWNER

13、COMMENTVNTNAME

14、pETM-30

15、COMMENTVNTAUTHORNAME

16、HüseyinBesir

17、COMMENTVNTOAUTHORNAME

18、UNKNOWN

19、COMMENTVNTREPLTYPE

20、PlasmidCOMMENTVNTEXTCHREPL

21、BacteriaFEATURESLocation/Qualifiersterminator1..129/gene="T7term"/vntifkey="43"/