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1、第28卷第4期中华中医药学刊Vo.l28No.42010年4月CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEApr.2010中肺合剂对小鼠Lewis肺癌肿瘤组织SurvivinCaspase-3表达的影响11,22122周晓芳,林能明,徐利,韩江敏,张会从,张如松(1.浙江省肿瘤医院,浙江杭州310022;2.浙江中医药大学药学院,浙江杭州310053)摘要:目的:观察中肺合剂对小鼠Lewis肺癌肿瘤组织survivin、caspase-3基因表达的影响。方法:取C57
2、BL/6小鼠50只,接种Lewis肺癌瘤株后,随机分阴性对照组(0.9%生理盐水),中肺合剂低、中、高剂量组-1-1-1-1(12.7g#kg、25.4g#kg、50.8g#kg),阳性对照组(环磷酰胺组,30mg#kg),每组10只。观察12天停药。无菌条件下取肿瘤组织,采用EnVision免疫组化方法测定中肺合剂对小鼠Lewis肺癌组织survivin、caspase-3蛋白表达的影响。结果:中肺合剂低、中、高给药组能不同程度下调Lewis肺癌小鼠肿瘤组织survivin蛋白的表达并上调caspase-3
3、蛋白的表达,且均以中剂量组最显著,与生理盐水组相比统计学上有非常显著差异(P<0.01)。结论:中肺合剂可通过下调survivin表达和上调caspase-3表达,从而促进肿瘤细胞凋亡,抑制肿瘤组织生长。关键词:中肺合剂;Lewis肺癌;EnVision法;survivin;caspase-3中图分类号:R285.5文献标识码:A文章编号:1673-7717(2010)04-0768-03TheEffectsofZhongfeiMixtureontheExpressionofSurvivinandCaspas
4、e-3inTumorTissuesfromtheMiceBearingLewisLungCarcinomaZHOUXiao-fang1,LINNeng-ming1,2,XULi2,HANJiang-min1,ZHANGHu-icong2,ZHANGRu-song2(1.ZhejiangCancerHospita,lHangzhou,310022,Zhejiang,China;2.CollegeofPharmaceuticalSciences,ZhejiangChineseMedicalUniversity,H
5、angzhou,310053,Zhejiang,China)Abstrac:tObjective:ToobservetheeffectsofZhongfeiMixture(ZM)ontheexpressionofsurvivinandcaspase-3intumortissuesfromthemicebearingLewislungcarcinoma.Methods:FiftyC57BL/6micebearingLewislungcarcinomaweredividedinto5groups:thesalin
6、econtrolgroup,thelow,midandhigh-dosegroupsofZMandCyclophosphamide(CTX)group.Tumortissueswereisolatedafter12daysfollowedbytheexpressiondetectionofsurvivinandcaspase-3intumortissuesbyEnVisiontwo-stepimmuno-histochemistrystaining.Results:ZMcoulddown-regulateth
7、eexpres-sionlevelofsurvivinandup-regulatingcaspase-3intumortissuesfromthemicebearingLewislungcarcinoma.Com-paredwithsalinecontrolgroup,theeffectofthemid-dosegroupwasthemostprominentandshowedsignificantlydiffer-entinstatistics(P<0.01).Conclusion:ZMcouldinhib
8、itproliferationandinduceapoptosisintumorcellsfromthemicebearingLewislungcarcinoma.Thepossiblemechanismisassociatedwithdown-regulatingtheexpressionlevelofsurvivinandup-regulatingcaspase-3intumortissuesr
