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1、·246·中国实验血液学杂志JournalofExperimentalHematology2003;11(3):246-250文章编号(ArticleID):1009-2137(2003)03-0246-05·论著·急性白血病病人骨髓细胞胞苷脱氨酶基因表达的研究孙志强,江 滨,赵晓 ,鲍 立,吴 彤,卢锡京,孔 圆,王德炳北京大学人民医院、血液病研究所,北京100044摘要 本研究的目的是测定胞苷脱氨酶(cytidinedeaminase,CDD)在正常骨髓细胞和不同病期急性白血病(AL)病人细胞内mRNA的表达水平,并探讨该酶与临床的关系。研究采用半定量逆转录2聚合酶链反应(RT2
2、PCR)检测83例AL病人(初治31例、复发难治29例、缓解23例)和15例正常人的骨髓细胞CDD表达,以CDD/β2actin大于或等于015定为高表达。结果显示,CDD基因表达量在初治和难治复发病例中分别为0.4905±0.28511和0.4969±0131802,高表达率分别为48.3%和51.7%;在完全缓解病例和正常人中分别为0.3338±0.23838和0.3193±0115390,高表达率分别为13.3%和15.4%;初治和难治复发病例的CDD基因表达量显著高于完全缓解病例和正常人(P<0.05),但初治与难治复发病例之间、完全缓解病例与正常人之间无显著差异(P>0.05
3、);难治复发病例中ALL的CDD表达量显著高于AML(0.7504±0.10939vs0.4336±0.33725;P<0.05);初治3例高表达中2例缓解,1例未缓解,5例低表达全部缓解;难治复发8例高表达中3例缓解,5例未缓解,10例低表达中4例缓解,6例未缓解,但未发现缓解率存在有显著性差异(P>0.05)。结论:CDD在AL的不同病期表达水平不同,但CDD高表达可能不是急性白血病的预后因素。关键词 急性白血病;胞苷脱氨酶;骨髓细胞;基因表达中图分类号 R733.71文献标识码 AExpressionofCytidineDeaminasemRNAinBoneMarrowCe
4、llsfromPatientswithAcuteLeukemiaSUNZhi2Qiang,JIANGBin,ZHAOXiao2Su,BAOLi,WUTong,LUXi2Jing,KongYuan,WANGDe2BingInstituteofHematology,People′sHospital,PekingUniversity,Beijing100044,ChinaAbstractTheaimofthestudywastodeterminetheexpressionofcytidinedeaminase(CDD)geneinbonemarrowcellsfrompatientswith
5、acuteleukemia(AL)andevaluatetherelationshipbetweenCDDexpressionandclinicalfeature.Semi2quantitativereversetranscriptionpolymerasechainreaction(RT2PCR)wasusedfordetectionofexpressionlevelofCDDmRNAinbonemarrowcellsfrom83patientswithacuteleukemiaandfrom15healthypeoplesascontrol.CDD/β2actinratio≥0.5
6、wasconsideredtobepositive.TheresultsshowedthatexpressionlevelsofCDDof31previouslyuntreatedpatientswerehigherthanthoseof23casesofALincompleteremissionandofnormalcontrols.ExpressionlevelsofCDDof29relapse/refractorypatientswerealsohigherthanthoseof23ALpatientsincompleteremissionandofnormalsubjects.
7、TheexpressionlevelsofCDDinrelapse/refractoryALLwerehigherthanthoseinAMLwhileexpressionlevelsofCDDwerenotcorrelatedwiththeoutcomeoftherapy.ItisconcludedthatthelevelofCDDmRNAexpressionvariesatthedifferentstageofacuteleukemia.T
