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1、752中国临床研究2010年9月第23卷第9期ChineseJournalofClinicalResearch,September2010,Vo123,No9论著血管内皮生长因子在急性髓系白血病骨髓上清、血浆中的表达及意义刘丽波宋艳秋徐公斌摘要!目的探讨血管内皮生长因子(VEGF)在急性髓系白血病(AML)骨髓上清、血浆中的表达及意义。方法选取23例初诊未治的AML患者作为研究对象。抽取枸橼酸钠抗凝的外周血2ml、肝素抗凝的骨髓3~5m,l离心后分别抽取血浆、骨髓上清,贮存于-
2、20∀冰箱待用。采用ELISA方法检测骨髓上清、血浆中VEGF的表达水平。骨髓上清VEGF对照选取8例非何杰金淋巴瘤无骨髓浸润患者,血浆VEGF对照选取5例健康体检者。结果初诊未治的AML患者骨髓上清、血浆VEGF的水平均明显高于对照组(P均<005);且骨髓上清水平明显高于血浆水平(P<005),二者呈正相关(r=0484,P=0031)。AML患者化疗后完全缓解组(n=6)与未缓解组(n=4)血浆VEGF水平均较治疗前明显升高(P均<005),但完全缓解组与未缓解组比较差异无统计学意义(P>
3、005)。化疗后完全缓解组与未缓解组骨髓VEGF水平均较治疗前略有下降,但差异无统计学意义(P>005);未缓解组与缓解组比较差异亦不显著(P>005)。结论初诊未治的AML骨髓上清、血浆VEGF的表达水平均明显升高,且骨髓上清明显高于血浆,具有诊断价值,可能为AML的治疗提供新的途径,但化疗后VEGF的变化不能作为判断疗效的指标。关键词!血管内皮生长因子;急性髓系白血病;骨髓上清;血浆中图分类号:R557文献标识码:A文章编号:1674-8182(2010)09-0752-02Expre
4、ssionanditssignificanceofvascularendothelialgrowthfactorinbonemarrowsupernatantsandplasmainpatientswithacutemyeloidleukemiaLIULibo,SONGYanqiu,XUGongbin.DepartmentofHemooncology,TheFourthHospital,JilinUniversity,Changchun130011,ChinaAbstract!Objecti
5、veTodeterminethelevelsofvascularendothelialgrowthfactor(VEGF)ofbonemarrowsupernatantsandplasmainpatientswithacutemyeloidleukemiaandassessitsclinicalimplication.MethodsTwentythreepatientswithnewlydiagnosedacutemyeloidleukemiawereservedasresearchobjects.
6、VEGFlevelsinbonemarrowsupernatantsandplasmaweremeasuredbysandwichELISA.ThebonemarrowsupernatantsinpatientswithnonHodgkin#slymphomaofnobonemarrowinfiltration(n=8)andplasmaofhealthysubjects(n=5)wererespectivelyservedascontrols.ResultsVEGFlevelsofprechemot
7、herapeuticbonemarrowsupernatantsandplasmainnewlydiagnosedpatientswithacutemyeloidleukemiaweresignificantlyhigherthanthoseincontrolgroups(allP<005),andVEGFlevelsofbonemarrowsupernatantswereobviouslyhigherthanthatofplasma(P<005).VEGFlevelinbonemarrowsuper
8、natantswascorrelatedwithVEGFlevelinplasma(r=0484,P=0031).TheplasmaVEGFlevelseitherincompleteremissiongroup(n=6)orinnonremissiongroup(n=4)aftertherapywereallobviouslyhigherthanthosebeforechemotherapy(allP<0
