资源描述：
《血小板源生长因子B及其受体_与支气管哮喘血管再生与重.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、164中华哮喘杂志(电子版)2009年6月第3卷第3期ChinJAsthma(ElectronicEdition),June2009,Vol.3,No.3论著血小板源生长因子B及其受体与支气管哮喘血管再生与重塑苏新明康健摘要!目的进一步明确支气管哮喘时血管相关因子血小板源生长因子B(PDGFB)和血小板源生长因子受体(PDGFR)在支气管哮喘小鼠模型气管及肺组织中的变化和作用。方法在建立小鼠支气管哮喘模型的基础上,应用免疫荧光染色和HE染色计数血管平滑肌细胞数量变化,应用逆转录多聚酶链反应(
2、RTPCR)方法检测气管和肺组织PDGFB及其受体(PDGFB/PDGFR)mRNA表达情况,应用Westernblot方法检测PDGFB/PDGFR蛋白水平。结果∀血管平滑肌细胞数量在支气管哮喘急性期减少,在慢性期又恢复正常;#应用RTPCR技术及琼脂糖凝胶电泳,PDGFBmRNA及PDGFRmRNA在正常气管未检测到,而在慢性延长期哮喘小鼠气管组织PDGFBmRNA显示出清晰的条带。周围肺组织在正常小鼠也表达PDGFBmRNA,哮喘小鼠肺组织表达增强,在慢性延长期表达明显增加。应用Westernblot蛋
3、白检测技术,哮喘小鼠气管及肺组织PDGFB及PDGFR蛋白表达情况与基因检测结果相一致。结论PDGFB和PDGFR基因与蛋白表达水平在支气管哮喘气管及肺组织均增加,特别在慢性延长期,PDGFB和PDGFR表达改变是血管平滑肌细胞数量变化的重要因素。PDGFB和PDGFR系统在支气管哮喘气道血管再生和重塑过程中可能起着重要的作用。关键词!支气管哮喘;血管平滑肌细胞;血小板源生长因子PlateletderivedgrowthfactorBanditsreceptorinairwayvascularangioge
4、nesisandremodelinginamurinemodelofbronchialasthmaSUXinming,KANGJian.InstituteofRespiratoryDisease,theFirstHospitalofChinaMedicalUniversity,Shenyang110001,ChinaAbstract!ObjectiveToelucidatetheroleofplateletderivedgrowthfactorB(PDGFB)andplateletderivedgrowthfac
5、torreceptor(PDGFR)invascularangiogenesisandremodelinginthemousemodelofasthma.MethodsAfterestablishmentofthemousemodelofbronchialasthma,immunofluorescenceandHEstainingwereusedtocountthenumberofvascularsmoothmusclecells(vSMC),thenreversetranscriptionpolymerasec
6、hainreaction(RTPCR)andwesternblotmethodswereusedtodetectmRNAandproteinlevelofPDGFBandPDGFRintracheaandlungtissues.Results∀Smoothmusclecellsdecreasedinacutephase,however,theyreturnedtonearlynormalinchronicstage;#PDGFBmRNAandPDGFRmRNAwerenotdetectedinnormaltrac
7、healtissue.Buttheywereincreasedintracheainchronicasthma.Theyshowedenhancedexpressionsinasthmalungtissuescomparedwiththoseinnormaltissues,especiallyinchronicphase.TheresultsofproteinlevelsusingwesternblotmethodwereconsistentwithmRNAexpression.ConclusionsPDGFBandP
8、DGFRmRNAandproteinshowedenhancedexpressionsinasthmatrachealandlungtissuescomparedwiththoseinnormaltissues,especiallyinchronicphase.ThisisamainfactorofvSMCchan
