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1、#250#胃肠病学和肝病学杂志2009年3月第18卷第3期ChinJGastroenterolHepato,lMar2009,Vo.l18,No.3实验性结肠炎p38丝裂原活化蛋白激酶表达的研究112331周进,吴正祥,杨九华,吴强,杨枫,周国胜1.安徽医科大学附属省立医院消化内科,安徽合肥230001;2.安徽医科大学第一附属医院检验科;3.安徽医科大学病理学教研室=摘要>目的观察三硝基苯磺酸(TNBS)诱导的大鼠结肠炎结肠组织p38MAPK表达与激活及大鼠血清TNF-A、IL-10水平变化,探讨p38MAPK在实验性结肠炎中的作用与机制。方法20只SD大鼠随机分为正常对照组(N
2、)与模型组(M),TNBS/乙醇法构建结肠炎模型,观察炎症活动指数(DAI)、大体形态损伤指数(CMDI)、组织学损伤指数(TDI),ELISA法检测血清TNF-A、IL-10水平,免疫组化染色检测大鼠结肠p38MAPK、p-p38MAPK表达。结果与N组相比,M组DAI、CMDI、TDI显著升高(P<0.01),血清TNF-A升高,IL-10水平下降(P<0.01),结肠组织p38MAPK表达增加(P<0.05),p-p38MAPK表达显著增加(P<0.01)。结论p38MAPK在实验性结肠炎的表达与激活均有明显增加,可能通过调节TNF-A、IL-10等细胞因子的表达参与结肠炎的
3、发病。=关键词>结肠炎;p38;丝裂原活化蛋白激酶;肿瘤坏死因子-A;白细胞介素-10中图分类号:R574.62文献标识码:A文章编号:1006-5709(2009)03-0250-03收稿日期:2008-10-20Thestudyonexpressionofp38mitogen-activatedproteinkinasesinexperimentalcolitis112331ZHOUJin,WUZhengxiang,YANGJiuhua,WUQiang,YANGFeng,ZHOUGuosheng1.DepartmentofGastroenterology,AnhuiProvin
4、cialHospitalAffiliatedtoAnhuiMedicalUniversity,Hefei230001;2.DepartmentofClinicalLaboratory,theFirstHospitalAffiliatedtoAnhuiMedicalUniversity;3.DepartmentofPatho-logy,AnhuiMedicalUniversity,China=Abstract>ObjectiveTostudytheexpressionandactivationofp38MAPKandthechangesofTNF-A,IL-10inTN-BS-ind
5、ucedcolitis,andtoexploretheeffectandmechanismofp38MAPKinexperimentalcolitis.MethodsTwentymaleSDratswererandomlydividedintonormalcontrolgroup(N)andmodelgroup(M).ColitiswasinducedbyintrarectaladministrationofTNBS/ethano.lThecolonicinflammationincludingdiseaseactivityindex(DAI),colonicmacroscopic
6、damageindex(CMDI)andtissuedamageindex(TDI)wereobserved,thelevelsofIL-10,TNF-AinserumweredetectedbyELISA,andtheexpressionsofp38MAPK,p-p38MAPKinthecolontissuesofratsweredetectedbyimmunohistochem-icalmethod.ResultsComparedwithnormalcontrolgroup,theDAI,CMDIandTDIwereincreasedsignificantly(P<0.01)i
7、nmodelgroup,thelevelofTNF-Ainserumincreased(P<0.01),andthelevelofIL-10reduced(P<0.01).Theexpressionsofp38MAPKincreased(P<0.05)incolonictissuesandp-p38MAPKincreasedsignificantly(P<0.01).ConclusionTheexpressionandactivationofp38MAPKincrea
