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1、64701991TheJapaneseSocietyofPathologyOriginalArticlesTransferrinReceptorsandSelectiveIronDepositioninPancreaticBCellsofIron-overloadedRatsJian-pingLu,KeikiHayashi,ShigeruOkada,andMichiyasuAwaiIronoverloadwasproducedinWistarratsbyrepeatedsitionintheBcellsof
2、thepancreaticisletshasbeenintraperitonealinjectionsofferricnitrilotriacetate(Fe3+-observedbothinthismodel(3,4)andincasesofhumanNTA)foronetosixmonths.Pancreatictissuesfromthesehemochromatosis(5).However,themechanismofthisiron-overloadedratsanduntreatedcontr
3、olswereex-selectiveirondepositionisobscure.aminedforinsulin(forBcells),glucagon(forAcells),Wereportedpreviouslythatmoretransferrinreceptorstransferrinreceptor(TfR),transferrin(Tf)andferritin(Ft)usingimmunohistochemicalmethods,andforironbyhisto-(TfR)weredis
4、tributedinisletcellsthaninacinarcellsofchemicalBerlinbluestaining.Intheisletsofiron-over-thepancreas(6).Therefore,wespeculatedthatTfR,loadedrats,increasedFtstainingappearedpriortodeposi-whichisaniron-transportingmembranestructuraltionofBerlinblue-stainable
5、iron,andthestaininginten-glycoprotein(7,8),mayplayaroleintheselectivedistri-sityofFtandironwasstrongerinBcellsthaninAcells.butionofiron.Basedonthishypothesis,weexaminedIntheisletsofuntreatedcontrolrats,thestainingintensitythedistributionofTfR,transferrin(T
6、f),ferritin(Ft)andofTfRwasstrongerinBcellsthaninAcells.TfRstainingBerlinblue-stainableirongranulesinpancreaticisletoftheisletswasweakeriniron-overloadedratsthaninthecellsofiron-overloadedanduntreatedcontrolrats.controls.Thesefindingssuggestthat1)ironuptake
7、byisletcellsinvivoisregulatedandmediatedbyTfR,2)intracytoplasmicFttransformsintostainableironiniron-MATERIALSANDMETHODSoverloadedrats,and3)predominanceofTfRexpressioninBcellsmayresultinselectivedepositionofironandOnehundredWistarratsweighing80-90g(1monthpr
8、edisposeBcellstodamageanddiabetesmellitusiniron-old)wereused.TheratswerepurchasedfromCharlesoverloadedrats.ActaPatholJpn41:647-652,1991.RiverJapanInc.(Kanagawa)anddividedintotwogroups.InGroupA(n=60),ironoverl
