非小细胞肺癌中EGFR和K_ras基因突变检测_金琳芳.pdf

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1、·1218·金琳芳，等非小细胞肺癌中EGFＲ和K－ras基因突变检测非小细胞肺癌中EGFＲ和K－ras基因突变检测金琳芳，齐晓薇，陆莲，浦勇，白瑞珍，秦艳，刘彦魁EGFＲandK－rasgenemutationinnon－smallcelllungcancerJinLinfang，QiXiaowei，LuLian，PuYong，BaiＲuizhen，QinYan，LiuYankuiDepartmentofPathology，TheAffiliatedHospitalofJiangnanUniversity(TheFourthHosp

2、italofWuxi)，JiangsuWuxi214000，China．【Abstract】Objective:ToinvestigatethegenemutatioofEGFＲandK－rasindifferenttypesofnon－smallcelllungcancer，andtherelationshipbetweenmutationsandpathologicalcharacteristics．Methods:GenesequencingwasusedtodetecttheEGFＲ(exons18，19，20，21)and

3、K－ras(codons12，13，61)genemutationstatusin98patientswithnon－smallcelllungcancer．Allpatientshadnotrecievedtreamentofgefitinib．Ｒesults:In98patients，EGFＲmutationratewas31．6%(31/98)，15patientshadNo．19deletionmutation，13patientshadNo．21L858Ｒpointmu-tation，3patientshadNo．20mu

4、tation，1patienthadNo．18mutation．1patienthadnotonlyNo．19deletionmutationbutalsoNo．20mutation．Mutationratewassiganificantlyhigherinadenocarcinomathaninadeo－squmaouscacino-ma，squmaouscellcacinomaandlargecellcarcinoma．Andwassiganificantlyhigherinfemalthaninmale，innon－smoki

5、ngthaninsmoking．Inadenocarcinoma，mutationratewashigherinpoorlydifferentiatedcarcinomathaninme-diumandwelldifferentiatedcarcinoma．K－rasmutationratewas21．4%(21/98)，whichwerefoundincodons12，13and61．Andmutationratewashigherinadenocarcinomathaninadeo－squmaouscacinoma，squmao

6、uscellcacino-maandlargecellcacinoma．NocorrelationwasfoundbetweenK－rasmutationsandthegender，thedifferentiationandsmokingstaus．Conclusion:ThedetectionrateofEGFＲmutationishigherinChinese．ThedetectionrateofK－rasmutationislowerinChinese．ThemutationinEGFＲandK－rasdoesnotprese

7、ntatthesametime．ThemutationinEGFＲofthenon－smallcelllungcancerisrelatedtogender，histologytypeanddifferentiation．ThemutationinK－rasisrelatedtohistologytype．【Keywords】non－smallcelllungcancer，EGFＲ，K－ras，mutationModernOncology2016，24(08):1218－1221【摘要】目的:探讨不同类型非小细胞肺癌的EGFＲ和K－

8、ras基因突变情况及其与肺癌相关临床病理特征的关系。方法:用厦门艾德ADxAＲMS试剂盒进行98例非小细胞肺癌患者肿瘤组织中EGFＲ(18，19，20，21外显子)基因和K－ras(12，13，61密码子)基因突变的检测。所有患者均未