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1、·临床研究·2008年3月第5卷第7期结核杆菌快速培养、耐药性的临床应用研究张涛,赵锦,佟立峰(深圳市南山区慢性病防治院,广东深圳518054)[摘要]目的:采用快速变色液体培养基进行结核杆菌培养及药物敏感性试验,建立一种适合基层实验室使用的快速培养及药敏试验方法。方法:①疑似肺结核痰标本200例镜检后分别接种于变色培养基和L-J培养基培养并观察结果。②35株结核杆菌采用变色培养法与罗氏绝对浓度法,65株结核杆菌采用变色培养法与罗氏比例法,分别进行药物敏感性试验。结果:变色培养基培养阳性率、污染率与L-J培养基无显
2、著性差异,培养阳性时间明显缩短;变色培养法与绝对浓度法药敏结果相比,符合率为60%,与比例法药敏结果相比,符合率为56.9%,药敏试验培养时间显著缩短。结论:采用快速变色培养基进行培养及药敏试验,提高了分枝杆菌快速检测水平,具有较好的基层推广价值。[关键词]分枝杆菌;快速培养;药敏试验[中图分类号]R446[文献标识码]A[文章编号]1673-7210(2008)03(a)-046-03Applicationofmycobacteriaquickcolouringliquidmediumandquickal-loc
3、hroicdrug-sensitivetestZHANGTao,ZHAOJin,TONGLi-feng(ChronicDiseaseHospitalofNanshanDistrict,ShenZhen518052,China)[Abstract]Objective:Usingquickcolouringliquidmediumtodobacillustuberculosiscultureandsusceptibilitytest,toestablisharapid,sensitve,specificandlow-c
4、ostmethodofrapidcultureanddrug-sensitivetestforbasiclevellab.Methods:①Sputumsamplesof200tuberculosis-likepatientswereinoculatedtoquickcoloringliquidmediumandL-JmediumrespectivelyaftermicroscopeinspectionbythicksmearingAcid-Faststain.②35strainsoftuberculosiswer
5、eappliedbymethodofquick,liquidcultureandconcentrationmethodofL-J,65strainswereappliedbymethodofquick,liquidcultureandproportionmethodofL-JtodetecttheirsusceptibilityofSM,INH,RFPandEB.Results:Forpostiverateandcontaminationrate,therewasnomarkeddifferencebetweenq
6、uickcolouringliquidmediumandL-Jmedium(P>0.05),buttherewasamarkeddifferenceinthetwogroupofdataonaveragegrowthtime(P<0.05);Theresultsofsuscepti-bilitytestbymethodofquick,liquidcultureandconcentrationmethodofL-Jshowthatcoincidenceis60%,Theresultsofquick,liquidcul
7、tureandproportionmethodshowthatcoincidenceis56.9%.Averagegrowthtimeofdrug-sensitivetestissignificantlyshortenthanroutinetest.Conclusion:Itcanimprovethelevelofrapidinspectiontousemycobacteriaquickcolouringliquidmediumtodomycobacteriacultureanddrug-sensitivetest
8、,andhaslocalclinicalapplicationandgeneralizingvalue.[Keywords]Liquidrapidmonitoring;Mycobacteria;Drugsensitivetest现将结核杆菌快速培养、耐药性的临床应用研究介绍如下:10月我院肺结核门诊肺结核病人痰标本培养分枝杆菌阳性病1材料与方法人100例(株
