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1、维生素E对小鼠日本血吸虫病肝纤维化的治疗作用及其机制作者：牛丽文，杨镇，肖亮，张爱龙，李亲健，乌剑利【关键词】，血吸虫病，日本；肝硬化；维生素E;氧化性应激；肝星状细胞；胶原I型[Abstract)AIM:ToinvestigatethetherapeuticeffectofvitaminE(VitE)andiismechanismsonliverfibrosisinducedbySchistosomajaponicuminfectioninmice.METHODS:Micewereinfecte
2、dwithSchistosomajaponicumcercariaepercutaneously,andweredividedinto5groups:normalcontrolgroup,modelgroup,andinterventiongroupswhichweretreatedwiththreedifferentdosesofVitE,150,50,and5mg/kg.That1ivefibrosisreachedgradeIIorabovewasconsideredtobeamarkero
3、ftherapeuticstart.Themicewerekilledattheendofthe8thweek.LiverlesionswereevaluatedusingHEandVGstaining.Immunohistochemistryforexsmoothmuscleactin(aSMA)asanactivatedhepaticstellatecel1(HSC)markerwasperformedtodetectandquantifyactivatedHSCbySPtechnique.T
4、hemalondialdehyde(MDA)contenlandsuperoxidedismutase(SOD)activityin1ivertissueweredeterminedbyspectrophotometricmethod.Thea1(1)procollagenmRNAexpressionwasmeasuredbyRTPCR.RESULTS:VitEreducedMDAcontent[(5.00±0.31)pmol/gvs仃1.66±1.84)pmol/g,P<0.05]andi
5、ncreasedSODactivity[(249.84±26.22)ykat/gvs(120.11±42.61)gkat/g,P<0.05]inlheIiverinmodeIgroupinadosedependenlmanner.Besides,VitEdecreasedthenumberofaSMApositivecelIs[(0.41±0.02)vs(0.68±0.02)，P&It;0.05]inadosedependentmannereFurther,VitEdiminishedthe
6、increasedcollagencontent[(0.23±0.01)vs(0.60±0.11),P<0.05]andinhibitedtheincreaseda1(I)procollagenmRNAexpression[(0.28士0.01)vs(0.85±0.15),P<0.05]inthe1iverinmodelgroup.CONCLUSION:VitEhasevidenttherapeuticeffectson1iverfibrosisresultedfromSchistos
7、omajaponicuminfectioninmice,andthemechanismsareassociatedwithanti1ipidperoxidation,inhibitionofHSCactivationandproliferation,andreductionofa1(I)procollagenmRNAexpressionandcollagenproductionbyVilE.【Keywords]Schistosomiasisjaponica;1ivercirrhosis;vitam
8、inE;oxidativestress;hepaticstellatecel1(HSC);collagentypeI【摘要】目的：探讨维生素E(VilE)抗小鼠日本血吸虫病肝纤维化作用及其机制.方法：用日本血吸虫尾蝴皮肤敷贴法感染小鼠，构建日本血吸虫病肝纤维化模型，以肝纤维化达II级或II级以上作为开始VitE治疗标志.实验分5组：正常对照组、模型组及VitE高、中、低剂量组(每日150,50,5mg/kg),8wk末处死动物，HE和VG染色对肝组织进行病理学检查，分光光度法检测肝
