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1、飞行时间质谱操作规程一，靶板清洗程序1.用丙酮冲洗靶板直到去除所有可见的样品残余2.用1%甲酸溶液超声10分钟3.用去离子水超声10分钟4.用分析纯丙酮超声10分钟5.用分析纯甲醇超声10分钟6.放入盒中，自然干燥二，样品制备基质选择及配置标准溶液本实验室现有基质如下：3-Indoleaceticacid（IAA），3-Hydroxypicolinicacid（HPA），Trihydroxyanthracene（DI），2,5-Dihydroxybenzoicacid（DHB），2',4',6'-Trihy
2、droxyacetophenonemonohydrate（THAP），2',6'-Dihydroxyacetophenone（DHAP），2-(4-Hydroxyphenylazo)benzoicacid(HABA)，根据测试人样品特点，参考相应文献报道，选择合适基质（以下表格仅供参考）Gentisicacid,Aldrich14,935-7Dissolve10mgin1mLofMostsamplessuchas2,5-Dihydroxybenzoicacid(DHB)mixedsolutionofacet
3、onitrileprotein,DNA,sugar,(C7H6O4:154.1)and0.1%TFA(2:3).lipidandsynthetichighpolymerFor1mgofhighpolymer,Polarsynthetichighdissolve10mgand1mgofpolymer(AllbecomeNaCl,LiClorKClinNa,LiorKaddedions.)demineralizedwater.Dissolve108mgand16mgofSinglestrandedDNA,amm
4、oniumcitratedibasicinaRNAof50merormore50%acetonitrilesolution.Dithranol,SigmaD-2773For1mgofhighpolymer,Non-polarsynthetic1,8-Dihydroxy-9[10H]-anthracenodissolve10mginchloroformorhighpolymerneTHF.(C14H10O3:226.2)For1mgofhighpolymer,Non-polarsyntheticdissolv
5、e10mgand1mgofhighpolymer(AllsilvertrifluoroacetateinbecomeAgaddedions.)chloroformorTHF.Remarks:1,由于本仪器主要用于合成化合物的表征，故以以上两种基质使用最为广泛。2,基质溶液应当在每次测试前配制，如需放置隔夜，请储存于闭光、低温条件（冰箱）三，点样Itisnecessarytousemethodsofdroppingtoasampleplatethataresuitablefordifferentsamples
6、ormatricesindividually.Thissectiondescribesthefollowingthreebasicmethods.Droppingmethod(1):Usuallyused.Droppingmethod(2):Usedtodropalargeamountofthesamesampletosamples.(Standardsampleintheautoanalysismode,etc.)Droppingmethod(3):Usedforsolidsamples.Dropping
7、method(1)1.Prepareanunusedcleansampleplate.2.Drop0.5μLofsamplesolutiontothespecifiedsamplewellusingapipette,etc.Takecarenotallowthepipetteendtotouchtheplate.3.Beforethecrystallizationofthesample(withinapproximately10seconds),dropamatrixsolutiononto0.5μLofs
8、amplesolution.4.Airdry.Caution:Exchangethepipettetipeverydropirrespectiveofmatricesorsamplesolutions.Thedroppingorderofamatrixandthatofasamplemaybereversedmutually.Droppingmethod(2)1.Prepareanunusedcleansampl
