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1、CAROBBEANGUMPreparedatthe53rdJECFA(1999)andpublishedinFNP52Add7(1999),supersedingspecificationspreparedatthe51stJECFA(1998),publishedinFNP52Add6(1998).ADI"notspecified",establishedatthe25thJECFAin1981.SYNONYMSLocustbeangum,carobgum,INSNo.410DEFINITIONPrimarilythegroundendospermofthes
2、eedsfromCeratoniasiliqua(L.)Taub.(Fam.Leguminosae)mainlyconsistingofhighmolecularweight(approximately50,000-3,000,000)polysaccharidescomposedofgalactomannans.Theseedsaredehuskedbytreatingthekernelswithdilutesulfuricacidathightemperatureorroastingthekernels,followedbymillingandscreeni
3、ngoftheseedstoobtaintheendosperm.Thegummaybepurifiedbywashingwithethanolorisopropanolordispersinginboilingwater,followedbyfiltering,evaporationanddrying.C.A.S.number9000-40-2DESCRIPTIONWhitetoyellowishwhite,nearlyodourlesspowderFUNCTIONALUSESThickener,stabilizer,emulsifierCHARACTERIS
4、TICSIDENTIFICATIONSolubility(Vol.4)InsolubleinethanolGelformationAddsmallamountsofsodiumborateTStoanaqueousdispersionofthesample;agelisformed.ViscosityTransfer2gofthesampleintoa400-mlbeakerandmoistenthoroughlywithabout4mlofisopropanol.Add,withvigorousstirring,200mlofwaterandcontinuet
5、hestirringuntilthegumiscompletelyanduniformlydispersed.Anopalescent,slightlyviscoussolutionisformed.Transfer100mlofthissolutionintoanother400-mlbeaker.Heatthemixtureinaboilingwaterbathforabout10minandcooltoroomtemperature.Thereisanappreciableincreaseinviscosity(differentiatingcarobbe
6、angumfromguargum).Gumconstituents(Vol.4)ProceedasdirectedunderGumConstituentsIdentification(Volume4)using100mgofthesampleinsteadof200mgand1-10µlofthehydrolysateinsteadof1-5µl.Usegalactoseandmannoseasreferencestandards.Theseconstituentsshouldbepresent.MicroscopicexaminationDisperseasa
7、mpleoftheguminanaqueoussolutioncontaining0.5%iodineand1%potassiumiodideonaglassslideandexamineundermicroscope.Carobbeangumcontainslongstretchedtubiformcells,separatedorslightlyinterspaced.TheirbrowncontentsaremuchlessregularlyformedthaninGuargum.(Guargumshowsclosegroupsofroundtopears
8、hapedcells.T
