免疫球蛋白基因组.ppt

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1、TheDiversityofImmunoglobulinYanWangSupervisor:AndrewCollinsCosupervisor:WilliamSewellImmunoglobulinStructuresHeavychainandlightchain(λandκ)ConstantregionClass:IgA,IgD,IgE,IgGandIgMVariableregion–AntigenbindingsiteGenerationofdiversitiesAIDN-RegionsPart1Immuno

2、globulingermlinegenerepertoirestudyIGHVgenerepertoireHighlypolymorphic226reportedgenesandallelicvariants104wasreportedinerror12putativenewpolymorphisms,3havebeensequencedConclusion:TheexistingIGHVgenerepertoireisfullofincorrectlyreportedsequences.IGKVgenerepe

3、rtoireanalysisCompilationofDatabase1863rearrangedIGKVsequencesfromtheEMBL66germlinegenesfromtheIMGTAlignmentsusingSmith-Watermanalgorithm.UnreportedIGKVallelesIGKValleleswithlownumberofunmutatedsequenceswerecollectedMultiplealignmentSharedmismatchesSequencing

4、fromgenomicDNAJunctionanalysisofIGKgenes435lowmutationsequences(<3IGKVmutations)wererealignedtodetermineIGKJgenesusingV-QUESTVJgeneuseageN-RegionResults5reportedgenesmaybetheresultofsequencingerrorsIGKV1D-13*01andIGKV1-37*01maybefunctionalinsomeindividualsUnr

5、eportedpolymorphisms:IGKV1-16*02andIGKV2-30*02.TheexistingIGKVrepertoireisrelativelycompleteandaccurateConclusionsNotonlyistherelittlediversityinthegermlinegenesofthekappalocus,butlittlejunctionaldiversityisgeneratedduringtheprocessofgenerearrangement.Theimmu

6、noglobulingenerepertoirecanthereforebecharacterisedashavingextraordinarydiversityintheheavychain,andlimiteddiversityinthelightchain.Part2SequencingimmunoglobulingenesfromallergicandparasitisedindividualsIgEandAllergyNaturalfunctionofIgE–ParasiteinfectionsIgEa

7、ndAllergicdiseaseAntigenselectioninIgETherelationshipbetweenIgEandIgG4UnderstandingIgEmutationsinPNGPNGIgEvsPNGIgGOtherFactorsExposuretopathogensAgeTwoQuestionsIsthereahigherlevelofmutationinantibodiesofPNGvillagers,comparedtoAustraliaurbanindividuals?Arehigh

8、ernumbersofmutationsseeninolderindividuals?SampleNameIgEIgGNo.ofIgAverageMutationNo.No.ofIgAverageMutationNo.Australia1812.85217.6A000001019.5A0021102418.8A006910.61815.1A007815.600PNG201