免疫球蛋白基因组

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1、TheDiversityofImmunoglobulinYanWangSupervisor:AndrewCollinsCosupervisor:WilliamSewellImmunoglobulinStructuresHeavychainandlightchain(λandκ)ConstantregionClass:IgA,IgD,IgE,IgGandIgMVariableregion–AntigenbindingsiteGenerationofdiversitiesAIDN-RegionsPar

2、t1ImmunoglobulingermlinegenerepertoirestudyIGHVgenerepertoireHighlypolymorphic226reportedgenesandallelicvariants104wasreportedinerror12putativenewpolymorphisms,3havebeensequencedConclusion:TheexistingIGHVgenerepertoireisfullofincorrectlyreportedsequen

3、ces.IGKVgenerepertoireanalysisCompilationofDatabase1863rearrangedIGKVsequencesfromtheEMBL66germlinegenesfromtheIMGTAlignmentsusingSmith-Watermanalgorithm.UnreportedIGKVallelesIGKValleleswithlownumberofunmutatedsequenceswerecollectedMultiplealignmentSh

4、aredmismatchesSequencingfromgenomicDNAJunctionanalysisofIGKgenes435lowmutationsequences(<3IGKVmutations)wererealignedtodetermineIGKJgenesusingV-QUESTVJgeneuseageN-RegionResults5reportedgenesmaybetheresultofsequencingerrorsIGKV1D-13*01andIGKV1-37*01may

5、befunctionalinsomeindividualsUnreportedpolymorphisms:IGKV1-16*02andIGKV2-30*02.TheexistingIGKVrepertoireisrelativelycompleteandaccurateConclusionsNotonlyistherelittlediversityinthegermlinegenesofthekappalocus,butlittlejunctionaldiversityisgenerateddur

6、ingtheprocessofgenerearrangement.Theimmunoglobulingenerepertoirecanthereforebecharacterisedashavingextraordinarydiversityintheheavychain,andlimiteddiversityinthelightchain.Part2SequencingimmunoglobulingenesfromallergicandparasitisedindividualsIgEandAl

7、lergyNaturalfunctionofIgE–ParasiteinfectionsIgEandAllergicdiseaseAntigenselectioninIgETherelationshipbetweenIgEandIgG4UnderstandingIgEmutationsinPNGPNGIgEvsPNGIgGOtherFactorsExposuretopathogensAgeTwoQuestionsIsthereahigherlevelofmutationinantibodiesof

8、PNGvillagers,comparedtoAustraliaurbanindividuals?Arehighernumbersofmutationsseeninolderindividuals?SampleNameIgEIgGNo.ofIgAverageMutationNo.No.ofIgAverageMutationNo.Australia1812.85217.6A000001019.5A0021102418.8A006910.61815.1A007815.600PNG201