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时间:2020-03-21
《氟吡汀对神经病理性疼痛大鼠的镇痛作用及其机制研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、中国疼痛医学杂志ChineseJournalofPainMedicine2014.20(4·213·doi:10.3969~.issn.1006—9852.2014.04.006氟吡汀对神经病理性疼痛大鼠的镇痛作用及其机制研究乌云尔德尼翁泽林冯涛张小沼(华中科技大学同济医学院附属协和医院疼痛科,武汉430022)摘要目的:探讨钾离子通道开放剂氟吡汀对神经病理性疼痛大鼠的镇痛作用及交感芽生的影响,为神经病理性疼痛药物治疗提供理论依据。方法:成年雄性SD大鼠18只,体重250~350g,按随机数字表法随机分为3组,每组6只:假手术组(S组),对照组(C组),氟
2、"kliT组(F组)。s组大鼠暴露腰5(L)脊神经,但不结扎。其余组大鼠暴露k脊神经并结扎,建立疼痛模型。s组和C组大鼠腹腔注射生理盐水2ml/kg,F组大鼠腹腔注射氟吡汀5m~/kg,并连续给药7d。各组大鼠术前3d及术后1、3、5、7d检测机械痛闽(pawwithdrawalpressurethreshold,PWPT)和缩足反射潜伏期(pawwithdrawallatency,PwL)。术后第7d,各组大鼠取手术侧L5背根神经节采用免疫荧光染色标记法检测背根神经节内篮状结构变化。结果:与s组比较,C组大鼠PWPT和PWL术后第1d起显著降低(P<0.
3、05),维持至术后第7d。与C组比较,F组大鼠术后第3d起PWPT和PWL明显提高,差异有统计学意义(P<0.05)。与C组比较,经过氟吡汀治疗后,F组大鼠术后第7d手术侧L背根神经节内篮状结构显著减少(P<0.05o结论:氟吡汀通过抑制背根神经节内交感芽生以减轻神经病理性疼痛。关键词神经病理性疼痛;氟吡汀;交感芽生;背根神经节ANALGESICEFFECTS0重’’LUPIRITlE0SPINALERVELIGArl’lU-IDUCEDNEURoPATHICPAININRATSWU.Yun.Er-De—Ni,WENGZe.Lin,FENGTao,ZHAN
4、GXiao.Ming△(DepartmentofPain,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430022)AbstractObjective:Toinvestigatetheeffectsofflupirtineonneuropathicpainandsympatheticsproutinginthedorsalrootganglionofratsafterspinalnerveligation(SNL).Methods:18he
5、althyadultmaleSprague·-Dawleyratsweighing250——350gwererandomlydividedintothreegroupswithsixratsineachgroup,includingshamgroup(groupS),controlgroup(groupC)andflupirtinegroup(groupF).TheneuropathicpainmodelwasestablishedbytheL5spinalnerveligation.IngroupS,theL5spinalnervewasexposedb
6、utnotligated.IngroupCandF,theL5spinalnervewasexposedandligated.TheratsingroupSandCreceivedsaline2ml/kgandtheratsingroupFreceivedflupirtine5mg/kgviaintraperitonealinjectiondailyforsevendaysafterthesurgery.Painthresholdwasestimatedbymeasuringpawwithdrawalpressurethreshold(PWPT)andla
7、tency(PWL)3daysbeforesurgery(baseline)and1,3,5,7daysaftersurgery.Theratsweresacrificedonthe7thdayaftersurgeryandtheL5dorsalrootganglionofallratswerecollectedtoobservethechangeofbasketformationbyimmunofluorescentstaining.Results:PWPTandPWLingroupCweresignificantlylowerthanthoseingr
8、oupSwithin1~7daysaftersurgeryfP<0
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