mir 200b和mir 200c在人胃癌患者血清中的表达及其临床意义.docx

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1、miR-200b和miR-200c在人胃癌患者血清中的表达及其临床意义研究生:贺利恒导师：曾希中文摘要目的：miRNA是一类短的非编码单链RNA,通过与靶mRNA结合來调节转录后水平的基因表达，促进mRNA的降解，抑制蛋白的翻译。研究显示miR-200b和miR-200c作为肿瘤抑制基因参与多种肿瘤的发生发展，然而miR-200b和miR-200c在胃癌患者血清中的作用未见报道，本文旨在探讨血清miR-200b和miR-200c在胃癌中表达改变及临床意义，判定其能否作为胃癌患者的预后标志。方法：收集96例胃癌患者术前血清标本和2

2、0例健康志愿者血液样品，提取血清RNA,运用qRT・PCR检测血清中miR-200b和miR-200c表达情况，分析miR-200b和miR-200c与胃癌患者临床病理参数以及预后之间的关系。结果：相对健康志愿者，血清miR-200b和miR-200c在胃癌患者中均显著下调；miR・200b和miR-200c的表达随着胃癌临床分期演进而下调(P<0.01),且有淋巴结转移的miR-200b和miR・200c的表达均显著低于无淋巴结转移组(P<0.01)；Spearman相关性分析显示miR-200b和miR-200c显著正相关；

3、Kaplan-Meier分析显示miR-200b或miR-200c表达越低，胃癌患者预后越差(P<0.01),而且miR-200b和miR-200c同时表达低的胃癌患者预后更差。结论：1.miR-200b和miR-200c在胃癌患者血清中表达下调，miR-200b和miR-200c下调与胃癌患者临床分期以及淋巴结转移相关。2.联合血清miR-200b和miR-200c可判定胃癌患者预后。关键词：miR-200b；miR-200c；血清；胃癌；临床意义Theexpressionandclinicalsignificanceofth

4、eserummiR-200bandmiR-200cinthegastriccancerAbstractPurpose:MicroRNAsaresmall,non-codingRNAsthatarecriticalregulatorsofvariousdiseasesincludingcancer,andmayrepresentanovelclassofcancerbiomarkers.RecentreportshavehighlightedthetumorsuppressoraspectsofmiR-200bandmiR-200

5、c.However,thelevelandclinicalrelevanceofcirculatingmiR-200bandmiR-200ctranscriptsinhumanserumofgastriccancerpatientsareunclear.ThepurposeofthisstudywastoidentifycirculatingmiR-200bandmiR-200ctranscriptsinhumanserumforuseasabiomarkerforstratificationandpredictionofpro

6、gnosisingastriccancer.Methods:WcanalyzedscrumlevelsofmiR-200bandmiR-200cin96patientswithdifferentstagesofgastriccancer・Bloodsampleswerecollectedbeforesurgeryandtherapy.Quantitativereversetranscription-polymerasechainreactionofcirculatingmiR-200bandmiR-200ctranscripts

7、wasperformeddirectlyinscrumtoimprovetheefficiencyofmiRNAassessment・Results:WefoundthatserummiR-200bandmiR-200cweredownregulatedinthegastriccancergroupandweresignificantlyassociatedwiththeclinicalstageandpresenceoflymphnodemetastasis.Furthermore,Kaplan-Meiersurvivalan

8、alysesrevealedpatientswithlowmiR-200bandmiR-200cexpressiondisplayedasignificantlypoorerprognosis.miR-200bandmiR-200cwerepositivecor