冠状病毒Coronavirusl论文-2018 Inactivation of Ebola virus and Middle East respiratory syndrome coronavirus in platelet concentrates and plasma by.pdf

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1、ORIGINALRESEARCHInactivationofEbolavirusandMiddleEastrespiratorysyndromecoronavirusinplateletconcentratesandplasmabyultravioletClightandmethyleneblueplusvisiblelight,respectively1223MarkusEickmann,UteGravemann,WiebkeHandke,FrankTolksdorf,322StefanReichenberg,ThomasH.Muller,€andAxelSeltsam

2、iddleEastrespiratorysyndromecoronavi-BACKGROUND:Ebolavirus(EBOV)andMiddleEastrus(MERS-CoV)andEbolavirus(EBOV)respiratorysyndromecoronavirus(MERS-CoV)haveareemerginginfectiousdiseasesthatcur-beenidentifiedaspotentialthreatstobloodsafety.ThisMrentlyarenotconsideredahighormoder-studyinvestig

3、atedtheefficacyoftheTHERAFLEX1,2atepriorityforbloodtransfusionsafetyandavailability.UV-PlateletsandTHERAFLEXMB-PlasmapathogenMostcasesofMERS-CoVinfectionhavearisenintheMid-inactivationsystemstoinactivateEBOVandMERS-CoVdleEast(particularlySaudiArabia)andintheRepublicofinplateletconcentrate

4、s(PCs)andplasma,respectively.3Korea,buttwodocumentedcaseswereobservedintrav-STUDYDESIGNANDMETHODS:PCsandplasma4elerstotheUnitedStates.ThissuggeststhatMERS-CoVwerespikedwithhightitersofcellculture–derivedEBOVmightcontinuetoemergeinWesterncountriesviatrans-andMERS-CoV,treatedwithvariousligh

5、tdosesofmissionbypersonstravelingtoorreturningfromaffectedultravioletC(UVC;THERAFLEXUV-Platelets)orareas.ThereisnoevidencetodatethatMERS-CoVmethyleneblue(MB)plusvisiblelight(MB/light;THERAFLEXMB-Plasma),andassessedforresidualviralinfectivity.ABBREVIATIONS:EBOV5Ebolavirus;MB5methyleneRESUL

6、TS:UVCreducedEBOV(4.5log)andMERS-blue;MB/light5methyleneblueincombinationwithCoV(3.7log)infectivityinPCstothelimitofdetection,visiblelight;MERS-CoV5MiddleEastrespiratorysyndromeandMB/lightdecreasedEBOV(4.6log)andMERS-coronavirus;PC(s)5plateletconcentrate(s);PI5pathogenCoV(3.3log)titer

7、sinplasmatonondetectablelevels.inactivation;RF5reductionfactor;TCID50550%tissueCONCLUSIONS:BothTHERAFLEXUV-Plateletscultureinfectiousdose.(UVC)andTHERAFLEXMB-Plasma(MB/light)1effectivelyreduceEBOVandMERS-CoVinfectivityinFromtheInstituteforVirology,PhilippsUniversity