冠状病毒Coronavirusl论文-2014 The Novel Human Coronavirus EMC Causes Disease in Macaques but not in Ferrets.pdf

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1、150:1,2014ESVP/ECVPProceedings201381EQUINEINFECTIOUSANAEMIAVIRUS-INDUCEDTHENOVELHUMANCORONAVIRUSEMCCAUSESPULMONARYINTERSTITIALDAMAGE:WHATABOUTDISEASEINMACAQUESBUTNOTINFERRETSAEROSOLTRANSMISSION?J.M.A.vandenBrand,L.Wiersma,S.L.Smits,L.Provacia,yzP.Bolfa*,J.L.Cadore,J.F.Mornex

2、,C.Catoi*,A.Gal*,V.S.Raj,A.D.M.E.Osterhaus,T.KuikenandzM.Taulescu*andC.LerouxB.L.Haagmans*PathologyDepartment,UniversityofAgriculturalSciencesandVeterinaryViroscienceLab,ErasmusMC,Rotterdam,TheNetherlandsyMedicine,Cluj-Napoca,Romania,InternalMedicineDepartment,VetAgroIntrodu

3、ction:In2012anovelhumancoronavirus(HCoV-EMC)zSup,Marcy-l’EtoileandUMR754INRAUniversityLyon1,Retrovirusandcausinglowerrespiratorytractinfectionwasdiscoveredinhumanpa-ComparativePathology,Francetients.Theclinicalcourseofthediseasewassimilartothatseeninse-Introduction:Equineinf

4、ectiousanaemia(EIA)isconsideredavereacuterespiratorysyndrome(SARS).However,thevirulenceandblood-bornediseaseprimarilytransmittediatrogenicallyorbyhae-pathogenesisofthevirusisnotyetknownandnoanimalmodelsformatophagousinsectsandthevirusinfectscellsofthemonocyte/humandiseasehav

5、ebeenestablished.macrophagelineage.AlternativeroutesoftransmissionhavenotMaterialsandMethods:Cynomolgusmacaquesandferretswerebeenexplored,althoughthereisevidencethatEIAwasspreadviainoculatedwithHCoV-EMC.At1or4daysafterinoculationthean-aerosolizedparticlesduringthe2006EIAviru

6、s(EIAV)outbreakinimalswerekilledandpathological,immunohistochemical,in-situhy-Ireland.bridization,virologicalandserologicalanalyseswereperformedonMaterialsandMethods:Haematoxylinandeosin-stainedsectionssamplesfromthoseanimals.Todeterminethepresenceoftherecep-oflungfrom77EIAV

7、seropositiveRomanianhorseswerescoredtorfortheHCoV-EMCdipeptidylpeptidase4(DDP4)indifferentbasedonlymphocyteinfiltration,(peri)bronchiolarinflammationcelltypesoftherespiratorytissue,immunohistochemicalanalysisandthicknessofthealveolarsepta.Immunolabellingforp26EIAVwasused.capsi

8、dproteinexpressionandsmoothmuscleactinwasperformed.Results:HCoV-EMCcausedmi