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1、JVetDiagnInvest14:97105(2002)FieldvalidationofacommercialblockingELISAtodifferentiateantibodytotransmissiblegastroenteritisvirus(TGEV)andporcinerespiratorycoronavirusandtoidentifyTGEV-infectedswineherdsSusyCarman,GaylanJosephson,BeverlyMcEwen,GrantMaxie,MioaraAntochi,KenEernisse,GopiNayar,P
2、atHalbur,GeneErickson,ErnstNilssonAbstract.AcommerciallyavailableblockingELISAwasanalyzedforitsabilitytoidentifyantibodiestoporcinecoronaviruses(transmissiblegastroenteritisvirus[TGEV]orporcinerespiratorycoronavirus[PRCV]),todifferentiateantibodiestoTGEVandPRCV,andtoidentifyTGEV-infectedher
3、ds.Nineserafromuninfectedpigs,34serafrom16pigsexperimentallyinfectedwithTGEV,andserafrom10pigsexperimentallyinfectedwithPRCVwereevaluatedusingboththeTGEV/PRCVblockingELISAandavirusneutralization(VN)assay.TheELISAwasnotconsistentlyeffectiveinidentifyingpigsexperimentallyinfectedwithTGEVuntil
4、21dayspostinfection.Serafrom100commercialswineherds(1,783sera;median15perherd)weresimilarlyevaluatedusingbothtests.ThirtyofthesecommercialherdshadaclinicalhistoryofTGEVinfectionandapositiveTGEVfluorescentantibodytestrecordedatnecropsywithinthelast35months,while70herdshadnohistoryofclinicalTG
5、EVinfection.TheblockingELISAandtheVNshowedgoodagreement(kappa0.84)forthedetectionofporcinecoronavirusantibody(TGEVorPRCV).Thesensitivity(0.933)oftheELISAtoidentifyTGEV-infectedherdswasgoodwhenconsideredonaherdbasis.TheELISAwasalsohighlyspecific(0.943)forthedetectionofTGEV-infectedherdswhenth
6、etestresultswereevaluatedonaherdbasis.Whenserafromspecificagegroupswerecompared,theELISAidentifiedagreaterproportion(0.83)ofpigsinherdswithTGEVantibodywhensucklingpigletswereused.Inrepeatabilityexperiments,theELISAgaveconsistentresultswhenthesameserawereevaluatedondifferentdays(kappa0.889)and
7、whenserawereevaluatedbeforeandafterheating(kappa0.888).TheblockingELISAwasdeterminedtobeusefulforherdmonitoringprogramsandcouldbeusedalonewithoutparalleluseoftheVNassayfortheassessmentoflargeswinepopulationsforthedetectionofTGEV-infectedherds.Transmissib
