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1、.I.J!lol.Rid.(1987)1%.963-966EvidenceforaCoiled-coilStructureintheSpikeProteinsofCoronavirusesTheaminoacidsequencesofthespikeproteinsfromthreedistantlyrelatedcoronaviruseshavebeendeducedfromcDNAsequences.IntheC-terminalhalf,anhomologyofabout3Oqbwasfound,whiletherewasnodetectable
2、sequenceconservationintheN-terminalregions.Hydrophobicheptadrepeatpatternsindicatedthepresenceoftwocr-heliceswitjhpredictedlengthsof100and50A,respectively.Itissuggestedthat,inthespikeoligomer.thesecl-helicesformacomplexcoiled-coil,resemblingthesupersecondarystructuresintwoothere
3、longatedmembraneproteins,thehaemagglutininofinfluenzavirusandthevariablesurfaceglycoproteinoftrypanosomes.CoronavirusesareenvelopedRNAviruseswithaAstretchof20to25hydrophobicresidues,foundsingle-strandedgenomeofpositivepolarity(SiddellneartheCterminus,mostprobablyservesasaetal.,1
4、983;Sturman&Holmes,1983).Theycausetransmembraneanchor.considerableeconomicaldamagebyinfectinglive-Aminoacidsequenceshavebeenalignedbythestockandotherdomesticanimals.Projectingfromfollowingprocedure.Initialaminoacidalignmentstheirsurfaceareunusuallylarge(-200A),petal-wereobt,aine
5、dbyFASTPanalysis(Lipman&shapedspikes.Theseso-calledpeplomersmediatePearson,1985).ThesealignmentshavebeenthebindingofvirionstothehostcellreceptorandextendedbyreiteratingFASTPwithnon-alignedareinvolvedinmembranefusion.Further,theyarepartsasquerysequenceandbyDTAGONcorn-consideredth
6、emaintargetsoftheprotectiveparison(Fig.l(a);Staden,1982).Theresultsareimmuneresponse(Sturman&Holmes,1983;summarizedinFigure2.MostconservationisCavanaghetal..1986a).observedintheC-terminalhalfoftheproteins.withEachpeplomerconsistsofadimerorpossiblyaoverallaminoacidhomologiesof35,
7、30andSS(!,t)rimerofthepeplomerprotein(Cavanagh,1983),aforTBV-FTPV,TBV-MHVandMHV-FTPV,res-glycoproteinof180,000to210,000M,(Sturman&pectively:about50%oftheaminoacidsubstit,u-Holmes.1983;Jacobsutal.,1986;Boyleetal.,tionsmaybeconsideredconservative(Dayhoffof1984).Thepeplomerproteins
8、ofmousehepatitisal.,1983).Tncontrast,wedidnot,f
