广西八一锰矿废弃地土壤蚕豆根尖细胞微核监测.pdf

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1、广西农业科学2010，41(9)：943—946GuarIgxiAgriculturalSciences·943·广西八一锰矿废弃地土壤蚕豆根尖细胞微核监测王任翔，一23，梁士楚1’2一，赖燕平1，2一，苏以丽1，谢彦军1(1广西师范大学生命科学学院，广西桂林541004；2广西环境工程与保护评价重点实验室，广西桂林541004；3广西师范大学珍稀濒危动植物生态与环境保护省部共建教育部重点实验室，广西桂林541004)摘要：采用蚕豆(ViciafabaL．)根尖细胞微核技术，研究广西八一锰矿废弃地4种作物(甘蔗、茶叶、柑橘和花生)种

2、植土壤的污染状况及安全性。结果表明，4种作物种植地土壤的蚕豆根尖细胞微核率均显著高于蒸馏水对照组，且上层(O～20cm)高于下层(20～40am)；上层土壤属中度污染，下层土壤属轻度污染；由土壤重金属污染引起的蚕豆根尖细胞微核畸变多达9种。说明广西八一锰矿废弃地土壤中含有遗传毒性物质，在其上不宜直接种植人畜食用的农作物，以免土壤重金属通过食物链进入躯体危害健康。关键词：蚕豆；细胞微核技术；锰矿废弃地；土壤污染；广西中图分类号：X53文献标识码：A文章编号：1002—8161(2010：109-0943—04Monitoringofs

3、oilpollutionofBayimanganeseminewasteland‘Guang‘‘。muclemofⅥ。fabaL．roottipcellsinuangxiuslnRmicronucleusc／aDaLroot-tipcellsOI’／J．WANGRen—xian91'2’3HANGShi-chul’2一，LAIYan-pin91’2·3SUYi-lil，XIEYan-junl(1CollegeofLifeScienceandTechnology，GuangxiNormalUniversity，Guilin，Guang

4、xi541004，China；2GuangxiKeyLaboratoryofEnvimnmentalEngineering，ProtectionandAssessment，Guilin，Guangxi541004，China；3KeyLaboratoryofEcologyofRareandEndangeredSpeciesandEnvironmentalProtectionofMinistryofEducation，GuangxiNormalUniversity，Guilin，Guangxi541004，China)Abstract

5、：ThepresentstudyaimedtomonitorthesoilpollutionandsafetyofBayimanganeseminewastelandinGuangxiusingmiemnucleusofViciafabaLroot-tipceHs．Soilsampleswerotakenfromsugarcane，tea，citrusandpeanutfields．TheViciafabaLroot—tiDcellstreatedwiththefoursoilsamplesshowedsignificantlyhi

6、ghermiemnueleusfrequen-cycomparedtothosetreatedwithdistilledwater．Themicronueleus矗equencyofViciafabaLroot—tiDceUstreatedwiththeuppersoil(O一20em)washigherthanthattreatedwiththelowersoil(20-40era)．ItreveMedthattheuppersoilWasmoderatelypollutedandthelowersoilWasslighflypo

7、lluted．NinekindsofmicmnucleusaberrationsinViciafabaL．root—tipceilswerefoundwhichwerocausedbyheavymetals．ItWasindicatedthatthesoilsofBayimanganeseminewastelandcontainedsomegeneticallytoxicsubstances．Theroforo．itWasnotsuitableforplantingcropdiroeflybothforanimalsandhuman

8、beinglesttheheavymetalsinsoilendangerpeople’Shealththroughfoodchain．Keywords：ViciafabaL；micronueleus；manganeseminewas